Modulation of Broiler Intestinal Changes Induced by Clostridium perfringens and Deoxynivalenol through Probiotic, Paraprobiotic, and Postbiotic Supplementation

Author:

de Souza Marielen123ORCID,Baptista Ana Angelita Sampaio2,Menck-Costa Maísa Fabiana2,Justino Larissa2,da Glória Eduardo Micotti4ORCID,Shimizu Gabriel Danilo5ORCID,Ferraz Camila Rodrigues6ORCID,Verri Waldiceu A.6ORCID,Van Immerseel Filip3,Bracarense Ana Paula Frederico Rodrigues Loureiro1ORCID

Affiliation:

1. Laboratory of Animal Pathology (LAP), Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Brazil

2. Laboratory of Avian Medicine (LAM), Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina 86057-970, Brazil

3. Livestock Gut Health Team (LiGHT), Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium

4. Biological Science Department, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba 13418-900, Brazil

5. Department of Statistics, Universidade Estadual de Londrina, Londrina 86057-970, Brazil

6. Laboratory of Pain, Inflammation, Neuropathy and Cancer, Department of General Pathology, Universidade Estadual de Londrina, Londrina 86057-970, Brazil

Abstract

Deoxynivalenol (DON) is a predisposing factor for necrotic enteritis. This study aimed to investigate the effects of a DON and Clostridium perfringens (CP) challenge on the intestinal morphology, morphometry, oxidative stress, and immune response of broilers. Additionally, we evaluated the potential of a Lactobacillus spp. mixture as an approach to mitigate the damage induced by the challenge. One-day-old broiler chickens (n = 252) were divided into seven treatment groups: Control, DON, CP, CP + DON, VL (DON + CP + viable Lactobacillus spp. mixture), HIL (DON + CP + heat-inactivated Lactobacillus spp. mixture), and LCS (DON + CP + Lactobacillus spp. mixture culture supernatant). Macroscopic evaluation of the intestines revealed that the CP + DON group exhibited the highest lesion score, while the VL and HIL groups showed the lowest scores. Microscopically, all Lactobacillus spp. treatments mitigated the morphological changes induced by the challenge. DON increased levels of reactive oxygen species (ROS) in the jejunum, and CP increased ROS levels in the jejunum and ileum. Notably, the Lactobacillus spp. treatments did not improve the antioxidant defense against CP-induced oxidative stress. In summary, a Lactobacillus spp. mixture, whether used as a probiotic, paraprobiotic, or postbiotic, exerted a partially protective effect in mitigating most of the intestinal damage induced by DON and CP challenges.

Funder

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Brasil

Conselho Nacional de Pesquisa e Desenvolvimento—Brasil

Publisher

MDPI AG

Reference73 articles.

1. Factors Affecting Intestinal Health in Poultry;Yegani;Poult. Sci.,2008

2. (2003). Regulation (EC) no 1831/2003 of the European Parliament and of the Council of 22 September 2003 on Additives for Use in Animal Nutrition (Standard No. No 1831/2003).

3. MAPA (2020). Instrução Normativa Nº 1, de 13 de Janeiro de 2020, Ministério da Agricultura Pecuária e Abastecimento.

4. Recent breakthroughs have unveiled the many knowledge gaps in Clostridium perfringens-associated necrotic enteritis in chickens: The first International Conference on Necrotic Enteritis in Poultry;Lyhs;Avian Pathol.,2016

5. Kulkarni, R.R., Gaghan, C., Gorrell, K., Sharif, S., and Taha-Abdelaziz, K. (2022). Probiotics as Alternatives to Antibiotics for the Prevention and Control of Necrotic Enteritis in Chickens. Pathogens, 11.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3