Abstract
In the context of the COVID-19 epidemic, enhancing the transport of analyte to a sensor surface is crucial for rapid detection of biomolecules since common conditions, including low diffusion coefficients, cause inordinately long detection times. Integrated microfluidic immunoassay chips are receiving increasing attention for their low sample volume and fast response time. We herein take advantage of asymmetric ICEO flow at a bipolar sinusoidal electrode to improve the rate of antibody binding to the reaction surface based on finite element modeling. Three different microfluidic cavities are proposed by changing the positions of the surface reaction area. We further investigate the relationship between binding enhancement and reaction surface positions, Damkohler number, and the voltage and frequency of the AC signal applied to the driving electrodes. Furthermore, the influence of the AC signal applied to the sinusoidal bipolar electrode on antigen–antibody-binding performance is studied in detail. Above all, the simulation results demonstrate that the microfluidic immune-sensor with a sinusoidal bipolar electrode could not only significantly improve the heterogeneous immunoassays but also enable efficient enhancement of assays in a selected reaction region within the micro-cavity, providing a promising approach to a variety of immunoassay applications, such as medical diagnostics and environmental and food monitoring.
Funder
National Natural Science Foundation of China
Basic Research Programs of Taicang, 2020
Subject
Electrical and Electronic Engineering,Mechanical Engineering,Control and Systems Engineering
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献