Author:
Song Chengchuang,Fang Xue,Yang Zhaoxin,Wang Qi,Meng Fantong,Chen Yaqi,Chen Junhao,Zhao Bei,Wang Yanhong,Fang Xingtang,Gu Lihong,Zhang Chunlei
Abstract
Though miRNAs have been reported to regulate bovine myoblast proliferation, but many miRNAs still need to be further explored. Specifically, miR-152 is a highly expressed miRNA in cattle skeletal muscle tissues, but its function in skeletal muscle development is unknown. Herein, we aimed to investigate the role of miR-152 in regulating bovine myoblast proliferation. Functionally, RT-qPCR, Western blotting, EdU assay, and flow cytometry detection results showed that miR-152 inhibited bovine myoblast proliferation. Mechanistically, we demonstrated transcription factor KLF6 was a target gene of miR-152 by means of bioinformatics software prediction and dual-luciferase report analysis, which had been demonstrated to be favorable for myoblast proliferation. Collectively, our research suggested that miR-152 inhibits bovine myoblast proliferation via targeting KLF6.
Funder
Program of Natural Science Foundation of the Jiangsu Higher Education Institutions of China
Subject
General Veterinary,Animal Science and Zoology
Cited by
5 articles.
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