A Metagenomic Survey of Wood Decay Fungi in the Urban Trees of Singapore
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Published:2023-04-10
Issue:4
Volume:9
Page:460
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ISSN:2309-608X
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Container-title:Journal of Fungi
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language:en
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Short-container-title:JoF
Author:
Hong Yan1ORCID, Tan Jhing Yein1ORCID, Xue Huiyu2, Chow Mei Lun1, Ali Mohamed2, Ng Arthur2, Leong Abigail2, Yeo Jeb2, Koh Shao Ming1, Tang Megan Shi Ying1, Lee Yan Yi1, Choong Amy Mei Fun3, Lee Serena Mei Lyn4, Delli Ponti Riccardo1, Chan Perry M.5, Lee Daryl2, Wong Jia Yih2, Mutwil Marek1ORCID, Fong Yok King2
Affiliation:
1. School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore 2. National Parks Board, 1 Cluny Road, Singapore Botanic Gardens, Singapore 259569, Singapore 3. Department of Biological Sciences, Faculty of Science, National University of Singapore, 14, Science Drive 4, Singapore 117543, Singapore 4. Singapore Botanic Gardens, Singapore 259569, Singapore 5. School of Applied Science, Nanyang Polytechnic, 180 Ang Mo Kio Avenue 8, Singapore 569830, Singapore
Abstract
Mature tropical urban trees are susceptible to root and trunk rot caused by pathogenic fungi. A metagenomic survey of such fungi was carried out on 210 soil and tissue samples collected from 134 trees of 14 common species in Singapore. Furthermore, 121 fruiting bodies were collected and barcoded. Out of the 22,067 OTUs (operational taxonomic units) identified, 10,646 OTUs had annotation information, and most were either ascomycetes (63.4%) or basidiomycetes (22.5%). Based on their detection in the diseased tissues and surrounding soils and/or the presence of fruiting bodies, fourteen basidiomycetes (nine Polyporales, four Hymenochaetales, one Boletales) and three ascomycetes (three species of Scytalidium) were strongly associated with the diseased trees. Fulvifomes siamensis affected the largest number of tree species surveyed. The association of three fungi was further supported by in vitro wood decay studies. Genetic heterogeneity was common in the diseased tissues and fruiting bodies (Ganoderma species especially). This survey identified the common pathogenic fungi of tropical urban trees and laid the foundation for early diagnosis and targeted mitigation efforts. It also illustrated the complexity of fungal ecology and pathogenicity.
Funder
The Ministry of National Development Research Fund/National Parks Board Singapore School of Biological Sciences, Nanyang Technological University Final Year Project
Subject
Plant Science,Ecology, Evolution, Behavior and Systematics,Microbiology (medical)
Reference38 articles.
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