Affiliation:
1. College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China
2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. College of Advanced Agricultural Sciences, Zhejiang Agriculture and Forestry University, Hangzhou 310007, China
Abstract
Anthracnose is one of the most widespread and destructive diseases in grapes. Grape anthracnose can be caused by various Colletotrichum species, such as Colletotrichum gloeosporioides and Colletotrichum cuspidosporium. In recent years, Colletotrichum aenigma was reported as a causal agent of Grape anthracnose in China and South Korea. Peroxisome is an important organelle in eukaryotes, which plays a very important role in the growth, development, and pathogenicity of several plant-pathogenic fungal species i, but it has not been reported in C. aenigma. In this work, the peroxisome of C. aenigma was labeled with a fluorescent protein, using green fluorescent protein (GFP) and red fluorescent protein (DsRED and mCherry) as reporter genes. Via Agrobacterium tumefaciens-mediated transformation (AtMT), two fluorescent fusion vectors to mark the peroxisomes, with GFP and DsRED, respectively, were introduced into a wild-type strain of C. aenigma. In the transformants, bright dots of green or red fluorescence in hyphae and spores could be seen in the strains labeled peroxisome. The nuclei labeled by the same method showed bright round fluorescent spots. In addition, we also combined fluorescent protein labeling with chemical staining to show the localization more clearly. The ideal peroxisome and nuclear fluorescence-labeled C. aenigma strain was obtained, which provided a reference for the study of its growth, development, and pathogenicity.
Funder
Zhejiang Key Research and Development Program
Three Agriculture and nine organizations” science and technology cooperation program of Zhejiang Province
Zhejiang Provincial Natural Science Foundation
Subject
Plant Science,Ecology, Evolution, Behavior and Systematics,Microbiology (medical)
Reference23 articles.
1. Literature review of plant anthracnose;Liu;J. Northwest For. Coll.,1995
2. Pathogen identification and pathogenicity analysis of grape ripe rot in Fujian;Lei;J. Fruit. Sci.,2014
3. Identification of the pathogen causing grape anthracnose in Yunnan and its pathogenicity;Deng;J. Plant. Prot.,2013
4. First Report of Anthracnose Caused by Colletotrichum aenigma on Actinidia arguta in China;Wang;Plant. Dis.,2019
5. First Report of Colletotrichum aenigma Causing Anthracnose of Grape (Vitis vinifera) in Korea;Kim;Plant Dis.,2021