Isocorydine Exerts Anticancer Activity by Disrupting the Energy Metabolism and Filamentous Actin Structures of Oral Squamous Carcinoma Cells
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Published:2024-01-09
Issue:1
Volume:46
Page:650-662
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ISSN:1467-3045
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Container-title:Current Issues in Molecular Biology
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language:en
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Short-container-title:CIMB
Author:
Zhou Qiaozhen1, Zhang Qianqian1, Liao Lingzi1ORCID, Li Qian1, Qu Huidan1, Wang Xinyu1, Zhou Ying1, Zhang Guangzeng1, Sun Mingliang1, Zhang Kailiang12, Zhang Baoping12
Affiliation:
1. Department (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, China 2. Key Laboratory of Dental Maxillofacial Reconstruction and Biological Intelligence Manufacturing, Lanzhou University, Lanzhou 730000, China
Abstract
Isocorydine (ICD) exhibits strong antitumor effects on numerous human cell lines. However, the anticancer activity of ICD against oral squamous cell carcinoma (OSCC) has not been reported. The anticancer activity, migration and invasion ability, and changes in the cytoskeleton morphology and mechanical properties of ICD in OSCC were determined. Changes in the contents of reactive oxygen species (ROS), the mitochondrial membrane potential (MMP), ATP, and mitochondrial respiratory chain complex enzymes Ⅰ–Ⅳ in cancer cells were studied. ICD significantly inhibited the proliferation of oral tongue squamous cells (Cal-27), with an IC50 of 0.61 mM after 24 h of treatment. The invasion, migration, and adhesion of cancer cells were decreased, and cytoskeletal actin was deformed and depolymerized. In comparison to an untreated group, the activities of mitochondrial respiratory chain complex enzymes I-IV were significantly decreased by 50.72%, 27.39%, 77.27%, and 73.89%, respectively. The ROS production increased, the MMP decreased by 43.65%, and the ATP content decreased to 17.1 ± 0.001 (mmol/mL); ultimately, the apoptosis rate of cancer cells increased up to 10.57% after 24 h of action. These findings suggest that ICD exerted an obvious anticancer activity against OSCC and may inhibit Cal-27 proliferation and growth by causing mitochondrial dysfunction and interrupting cellular energy.
Funder
Lanzhou City Science and Technology Program Fundamental Research Funds for the scientific research fund of the School (Hospital) of Stomatology Lanzhou University
Subject
Microbiology (medical),Molecular Biology,General Medicine,Microbiology
Reference43 articles.
1. Imbesi Bellantoni, M., Picciolo, G., Pirrotta, I., Irrera, N., Vaccaro, M., Vaccaro, F., Squadrito, F., and Pallio, G. (2023). Oral Cavity Squamous Cell Carcinoma: An Update of the Pharmacological Treatment. Biomedicines, 11. 2. Tongue cancer: A discrete oral cavity subsite;Shetty;Oral Oncol.,2019 3. Classification of GLOSSECTOMIES: Proposal for tongue cancer resections;Ansarin;Head Neck,2019 4. Navarro-Orcajada, S., Vidal-Sánchez, F.J., Conesa, I., Escribano-Naharro, F., Matencio, A., and López-Nicolás, J.M. (2023). Antiproliferative Effects in Colorectal Cancer and Stabilisation in Cyclodextrins of the Phytoalexin Isorhapontigenin. Biomedicines, 11. 5. Yang, D., Zhang, H., Wu, J., Ma, R., Li, Z., Wang, K., and Yang, F. (2019). The role of chamaejasmine in cellular apoptosis and autophagy in MG-63 cells. Biosci. Rep., 39.
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