Affiliation:
1. Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan
2. Joint Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan
3. Faculty of Veterinary Medicine, Airlangga University, Mulyorejo, Surabaya 60115, Indonesia
Abstract
The majority of myxosporean species (Cnidaria: Myxozoa) of the genera Myxobolus (35 species), Henneguya (8 species), and Myxidium (9 species) from freshwater or brackish fish in Japan were recorded more than 30 years ago (accumulatively 81.1% [43/53]). The re-discovery and molecular–genetic characterization of these species is a current research priority. During our myxosporean survey in Japanese freshwater fish, we detected three species that had never been recorded in Japan, but in the Russian Far East (Sakhalin Island, and Maritime Province): Myxobolus tribolodonus sp. n., forming cysts in the gills of Tribolodon sachalinensis (syn. M. marinus sensu Aseeva, 2000; M. marinus sensu Sokolov et Frolova, 2015, recorded from the gills of Pseudaspius (syn. Tribolodon) spp.); Henneguya pungitii Achmerov, 1953, forming cysts in the subcutis of external skin and buccal submucosa of Pungitius sinensis; and Myxidium salvelini Konovalov et Shulman, 1966, in the urinary bladder of Oncorhynchus masou ishikawae. These new isolates were characterized by integrated taxonomic approaches, i.e., myxospore morphology and molecular–genetic characterization of the small subunit ribosomal RNA gene (SSU rDNA). These new isolates were phylogenetically differentiated from any species whose SSU rDNA sequences were deposited in the DNA databases, and concurrently compared with recorded species based on classical morphological criteria. All three species were differentiated from myxosporeans previously recorded in Japan, indicating new distribution records out of the Russian Far East. For reliable species identification, accumulation of at least SSU rDNA sequences of known species worldwide is critically important.
Funder
Grant-in-Aid for Food Science and Research 2019 from The Toyo Suisan Foundation (HS), JSPS Kakenhi
The Yanmar Environmental Sustainability Support Association
Grant-in-Aid for International Collaboration Research in Asia 2023 from the Heiwa Nakajima Foundation
Reference118 articles.
1. Okamura, B., Gruhl, A., and Bartholomew, J.L. (2015). Myxozoan Evolution, Ecology and Development, Springer International Publishing.
2. A review of the myxosporean genus Kudoa Meglitsch, 1947, and its impact on the international aquaculture industry and commercial fisheries;Moran;Aquaculture,1999
3. Recent advances in our knowledge of the Myxozoa;Kent;J. Eukaryot. Microbiol.,2001
4. Carvalho, E.D., David, G.S., and Silva, R.J. (2012). Transmission biology of the Myxozoa. Health and environment in Aquaculure, InTech.
5. Extensive uncharted biodiversity: The parasite dimension;Okamura;Integr. Comp. Biol.,2018