A Multi-Faceted Binding Assessment of Aptamers Targeting the SARS-CoV-2 Spike Protein

Author:

Civit Laia1ORCID,Moradzadeh Nima2ORCID,Jonczyk Anna2ORCID,Neckermann Patrick3ORCID,Asbach Benedikt3ORCID,Peterhoff David34ORCID,Wagner Ralf34,Famulok Michael25,Mayer Günter25,Kjems Jørgen16ORCID,Valero Julián16ORCID

Affiliation:

1. Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus, Denmark

2. Life and Medical Sciences (LIMES), University of Bonn, 53121 Bonn, Germany

3. Institute of Medical Microbiology and Hygiene, Molecular Microbiology (Virology), Regensburg University, 93053 Regensburg, Germany

4. Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, 93053 Regensburg, Germany

5. Center of Aptamer Research & Development, University of Bonn, 53121 Bonn, Germany

6. Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, Denmark

Abstract

The COVID-19 pandemic has underscored the critical need for the advancement of diagnostic and therapeutic platforms. These platforms rely on the rapid development of molecular binders that should facilitate surveillance and swift intervention against viral infections. In this study, we have evaluated by three independent research groups the binding characteristics of various published RNA and DNA aptamers targeting the spike protein of the SARS-CoV-2 virus. For this comparative analysis, we have employed different techniques such as biolayer interferometry (BLI), enzyme-linked oligonucleotide assay (ELONA), and flow cytometry. Our data show discrepancies in the reported specificity and affinity among several of the published aptamers and underline the importance of standardized methods, the impact of biophysical techniques, and the controls used for aptamer characterization. We expect our results to contribute to the selection and application of suitable aptamers for the detection of SARS-CoV-2.

Funder

Carlsberg

Danish National Research Foundation to the Center for Cellular Signal Patterns

European Union H2020-FETOPEN

Federal Ministry of Education and Research

German Research Council

Publisher

MDPI AG

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