Magnetic SERS Strip Based on 4-mercaptophenylboronic Acid-Modified Fe3O4@Au for Active Capture and Simultaneous Detection of Respiratory Bacteria
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Published:2023-01-31
Issue:2
Volume:13
Page:210
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ISSN:2079-6374
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Container-title:Biosensors
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language:en
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Short-container-title:Biosensors
Author:
Li Jingfei1, Chen Jin12, Dai Yuwei1, Liu Zhenzhen1, Zhao Junnan1, Liu Shuchen3, Xiao Rui1
Affiliation:
1. Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China 2. Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China 3. Beijing Institute of Radiation Medicine, Beijing 100850, China
Abstract
The rapid diagnosis and detection of respiratory bacteria at the early stage can effectively control the epidemic spread and bacterial infection. Here, we designed a rapid, ultrasensitive, and quantitative lateral flow immunoassay (LFA) strip for simultaneous detection of respiratory bacteria S. aureus and S. pneumoniae. In this assay, the surface enhanced Raman scattering (SERS) tags were designed through combining magnetite Raman enhancement nanoparticle Fe3O4@Au/DTNB and recognition element 4-mercaptophenylboronic acid (4-MPBA). Further, 4-MPBA could capture multiple bacteria in a complex environmental solution. Based on the strategies, Fe3O4@Au/DTNB-mediated magnetic enrichment and 4-MPBA-mediated universal capture capabilities improved the detection sensitivity, the limits of detection for S. aureus and S. pneumoniae were as low as 8 and 13 CFU mL−1, respectively, which were more sensitive than those of colloidal gold method. The Fe3O4@Au/DTNB/Au/4-MPBA-LFA also exhibited good reproducibility, excellent specificity, and high recovery rates in sputum samples, indicating its potential application in the detection of respiratory bacteria samples.
Funder
National Key Research and Development Program of China the Major Project Youth Cultivation Project
Subject
Clinical Biochemistry,General Medicine,Analytical Chemistry,Biotechnology,Instrumentation,Biomedical Engineering,Engineering (miscellaneous)
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