SMART: A Swing-Assisted Multiplexed Analyzer for Point-of-Care Respiratory Tract Infection Testing

Author:

Zhang Li1,Wang Xu1,Liu Dongchen1,Wu Yu1,Feng Li2,Han Chunyan2,Liu Jiajia1,Lu Ying13,Sotnikov Dmitriy V.4ORCID,Xu Youchun13,Cheng Jing13

Affiliation:

1. Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China

2. CapitalBiotech Technology, Beijing 101111, China

3. National Engineering Research Center for Beijing Biochip Technology, Beijing 102200, China

4. A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia

Abstract

Respiratory tract infections such as the ongoing coronavirus disease 2019 (COVID-19) has seriously threatened public health in the last decades. The experience of fighting against the epidemic highlights the importance of user-friendly and accessible point-of-care systems for nucleic acid (NA) detection. To realize low-cost and multiplexed point-of-care NA detection, a swing-assisted multiplexed analyzer for point-of-care respiratory tract infection testing (SMART) was proposed to detect multiple respiratory tract pathogens using visible loop-mediated isothermal amplification. By performing hand-swing movements to generate acceleration force to distribute samples into reaction chambers, the design of the SMART system was greatly simplified. By using different format of chips and integrating into a suitcase, this system can be applied to on-site multitarget and multi-sample testing. Three targets including the N and Orf genes of SARS-CoV-2 and the internal control were simultaneously analyzed (limit of detection: 2000 copies/mL for raw sample; 200 copies/mL for extracted sample). Twenty-three clinical samples with eight types of respiratory bacteria and twelve COVID-19 clinical samples were successfully detected. These results indicate that the SMART system has the potential to be further developed as a versatile tool in the diagnosis of respiratory tract infection.

Funder

National Natural Science Foundation of China

Emergency Key Program of Guangzhou Laboratory

Beijing Nova Program 2020 and Beijing Lab Foundation and Russian Foundation for Basic Research

Publisher

MDPI AG

Subject

Clinical Biochemistry,General Medicine,Analytical Chemistry,Biotechnology,Instrumentation,Biomedical Engineering,Engineering (miscellaneous)

Reference36 articles.

1. WHO (2022, December 26). The Top 10 Causes of Death, Available online: https://www.who.int/news-room/fact-sheets/detail/the-top-10-causes-of-death.

2. Public health emergencies of international concern: A historic overview;Osman;J. Travel Med.,2020

3. A novel coronavirus outbreak of global health concern;Wang;Lancet,2020

4. Point-of-care testing detection methods for COVID-19;Song;Lab Chip,2021

5. COVID-19 Point-of-Care Diagnostics: Present and Future;Valera;ACS Nano,2021

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