Abstract
The three-dimensional structure of protein is determined by analyzing diffraction data collected using X-ray beams. However, X-ray beam can damage protein crystals during data collection, lowering the quality of the crystal data. A way to prevent such damage is by treating protein crystals with cryoprotectants. The cryoprotectant stabilizes the protein crystal and prevents lowering the quality of the diffraction data. Many kinds of cryoprotectants are commercially available, and various treatment methods have also been reported. However, incorrect selection or treatment of such cryoprotectants may lead to deterioration of crystal diffraction data when using X-ray beams.
Funder
the Bio & Medical Technology Development Program and ICT
Subject
Inorganic Chemistry,Condensed Matter Physics,General Materials Science,General Chemical Engineering
Reference64 articles.
1. Über das Milzvenenblut;Funke;Z. Rat. Med.,1851
2. Die Chemismus in der Thierischen Organization;Hunefeld,1840
3. Ueber die Eiweisskörper verschiedener Oelsamen
4. The proteids of the Brazil nut;Osborne;Am. Chem. J.,1891
5. Protein data bank;Nat. New Biol.,1971
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献