Flavonoid Metabolome-Based Active Ingredient Mining and Callus Induction in Catalpa bungei C. A. Mey

Author:

Zeng Xiaofeng12,Wang Xiao12,Zeng Yanling12ORCID,Hou Jinbo13,Liu Zhiming4

Affiliation:

1. Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China

2. Key Laboratory of Non-Wood Forest Products of State Forestry Administration, Central South University of Forestry and Technology, Changsha 410004, China

3. Anhui Hongsen Hi Tech Forestry Co., Ltd., Bozhou 236814, China

4. Department of Biology, Eastern New Mexico University, Portales, NM 88130, USA

Abstract

Catalpa bungei C. A. Mey is a unique and precious multi-purpose tree species that possesses great timber-related, ornamental and medicinal values. In this study, MS, N6 and DKW were used as basic media, and different concentrations of 6-BA and NAA were added for callus induction. The induction rate and total flavonoid content of callus tissue showed that the best callus induction medium was DKW + 2.0 mg·L−1 6-BA + 0.5–1.0 mg·L−1 NAA. The leaves of different bark phenotypes of C. bungei C. A. Mey and the callus tissue extracted from young leaves of C. bungei C. A. Mey were used as experimental materials to construct metabolomic profiles of widely targeted flavonoids. Based on the metabolomic databases, the predominant flavonoids were screened from the callus tissues. Eight flavonoid metabolites increased in callus, and diosmetin-7-O-rutinoside (diosmin) was the flavonoid constituent with the shortest retention time, most efficient detection and best medicinal functions among these 8. The optimal medium for callus induction was supplemented with different concentrations of elicitors (salicylic acid SA and yeast extract YE). The optimal elicitor and the amount to be added were determined by analyzing the induction rate of callus, as well as the total contents of flavonoids and diosmin. The addition of SA and YE in appropriate amounts increased the total flavonoid content in the callus, but only the addition of YE promoted the formation of diosmin in the callus. The optimal medium formulation to promote the formation of callus was DKW + 2.0 mg·L−1 6-BA + 1.0 mg·L−1 NAA + 30 g·L−1 sucrose + 6.8 g·L−1 agar + 10 μmol·L−1 SA. The medium formulation to optimally increase the content of geraniol glucoside was DKW + 2.0 mg·L−1 6-BA + 1.0 mg·L−1 NAA + 30 g·L−1 sucrose + 6.8 g·L−1 agar + 200 mg·L−1 YE. The results of the present study will provide a scientific basis for the subsequent increase in the content of the active components of the suspension cells via the addition of elicitors, and for the production of diosmin in factory settings.

Funder

National Science and Technology Achievement Promotion Project of National Forestry and Grassland Administration

Hunan Province Forestry Science and Technology Innovation Project

Publisher

MDPI AG

Subject

Forestry

Reference33 articles.

1. Xu, H., Hu, G., Dong, J., Wei, Q., Shao, H., and Lei, M. (2014). Antioxidative Activities and Active Compounds of Extracts from Catalpa Plant Leaves. Sci. World J., 857982.

2. Chemical components from the seeds of Catalpa bungei and their inhibitions of soluble epoxide hydrolase, cholinesterase and nuclear factor kappa B activities;Tang;RSC Adv.,2016

3. Zhuo, Z.-Q. (2014). Study on Growth Law of Catalpa bungei and Accumulation Law of the Total Flavone in Catalpa bungei Leaf Blade. [Master’s Thesis, Northwest A&F University].

4. Xu, H. (2015). Studies on Chemical Constituents and Biological Activities of the catalpa bungei C.A. Mey. Leaves and Seeds. [Doctor’s Thesis, Northwest A&F University].

5. The ways of producing secondary metabolites via plant cell culture;Gu;Plant Physiol. J.,2013

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