Abstract
A relevant alternative to enamel matrix derivatives from animal origin could be the use of synthetic amelogenin-derived peptides. This study aimed to assess the effect of a synthetic amelogenin-derived peptide (ADP-5), alone or included in an experimental gellan–xanthan hydrogel, on periodontal cell behavior (gingival fibroblasts, periodontal ligament cells, osteoblasts and cementoblasts). The effect of ADP-5 (50, 100, and 200 µg/mL) on cell metabolic activity was examined using Alamar blue assay, and cell morphology was assessed by confocal imaging. An experimental gellan–xanthan hydrogel was then designed as carrier for ADP-5 and compared to the commercial gel Emdogain®. Alizarin Red was used to determine the periodontal ligament and cementoblasts cell mineralization. The inflammatory profile of these two cells was also quantified using ELISA (vascular endothelial growth factor A, tumor necrosis factor α, and interleukin 11) mediators. ADP-5 enhanced cell proliferation and remineralization; the 100 µg/mL concentration was more efficient than 50 and 200 µg/mL. The ADP-5 experimental hydrogel exhibited equivalent good biological behavior compared to Emdogain® in terms of cell colonization, mineralization, and inflammatory profile. These findings revealed relevant insights regarding the ADP-5 biological behavior. From a clinical perspective, these outcomes could instigate the development of novel functionalized scaffold for periodontal regeneration.
Funder
Institut Carnot Chimie Balard de Montpelier
InterCarnot Ingénierie@Lyon de Lyon
Subject
Biomedical Engineering,Biomaterials
Reference59 articles.
1. Periodontal diseases;Kinane;Nat. Rev. Dis. Primer,2017
2. The Future of Periodontal-Systemic Associations: Raising the Standards;Bartold;Curr. Oral Health Rep.,2017
3. Scientific evidence on the links between periodontal diseases and diabetes: Consensus report and guidelines of the joint workshop on periodontal diseases and diabetes by the International Diabetes Federation and the European Federation of Periodontology;Sanz;Diabetes Res. Clin. Pract.,2018
4. Minervini, G., Basili, M., Franco, R., Bollero, P., Mancini, M., Gozzo, L., Romano, G.L., Marrapodi, M.M., Gorassini, F., and D’Amico, C. (2022). Periodontal Disease and Pregnancy: Correlation with Underweight Birth. Eur. J. Dent., 12–14.
5. Periodontitis and preeclampsia in pregnancy: A systematic revieuw and meta-analysis;Le;Matern. Child Health J.,2022
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献