Voghera Sweet Pepper: A Potential Ally against Oxidative Stress and Aging

Author:

Gola Federica1,Gaiaschi Ludovica1ORCID,Roda Elisa12ORCID,De Luca Fabrizio1ORCID,Ferulli Federica3,Vicini Riccardo3,Rossi Paola1,Bottone Maria Grazia1ORCID

Affiliation:

1. Department of Biology and Biotechnology, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy

2. Laboratory of Clinical and Experimental Toxicology, Pavia Poison Centre—National Toxicology Information Centre, Toxicology Unit, Istituti Clinici Scientifici Maugeri, IRCCS Pavia, 27100 Pavia, Italy

3. Bio Basic Europe S.R.L., Via Taramelli 24, 27100 Pavia, Italy

Abstract

In the present study, the potential functional properties of the extracts from the edible part of Capsicum annuum L. var. Peperone di Voghera (VP) were studied. The phytochemical analysis revealed a high amount of ascorbic acid, paralleled by a low carotenoid content. Normal human diploid fibroblasts (NHDF) were chosen as the in vitro model models to investigate the effects of the VP extract on oxidative stress and aging pathways. The extract of Carmagnola pepper (CP), another important Italian variety, was used as the reference vegetable. The cytotoxicity evaluation was performed firstly, using a 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay, while the VP potential antioxidant and antiaging activity was investigated by immunofluorescence staining focusing on specifically selected proteins. The MTT data revealed the highest cell viability at a concentration of up to 1 mg/mL. The immunocytochemical analyses highlighted an increased expression of transcription factors and enzymes involved in redox homeostasis (Nrf2, SOD2, catalase), improved mitochondrial functionality, and the up-regulation of the longevity gene SIRT1. The present results supported the functional role of the VP pepper ecotype, suggesting a feasible use of its derived products as valuable food supplements.

Funder

University of Pavia: Fondi Ricerca Giovani

MUR—M4C2 1.5 of PNRR

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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