Dipotassium Glycyrrhizininate Improves Skin Wound Healing by Modulating Inflammatory Process

Author:

Leite Camila dos Santos12,Bonafé Gabriel Alves2,Pires Oscar César3,Santos Tanila Wood dos1,Pereira Geovanna Pacciulli4,Pereira José Aires4,Rocha Thalita5,Martinez Carlos Augusto Real4ORCID,Ortega Manoela Marques2ORCID,Ribeiro Marcelo Lima1ORCID

Affiliation:

1. Laboratory of Immunopharmacology and Molecular Biology, São Francisco University Medical School (USF), Bragança Paulista, São Paulo 12916-900, Brazil

2. Laboratory of Cell and Molecular Tumor Biology and Bioactive Compounds, São Francisco University Medical School (USF), Bragança Paulista, São Paulo 12916-900, Brazil

3. Laboratory of Pharmacology, Taubaté University (UNITAU), Taubaté, São Paulo 12030-180, Brazil

4. Department of Surgery and Proctology, São Francisco University (USF), Bragança Paulista, São Paulo 12916-900, Brazil

5. Postgraduate Program in Biomaterials and Regenerative Medicine, Faculty of Medical Sciences and Health, Pontifical Catholic University of São Paulo, São Paulo 05014-901, Brazil

Abstract

Wound healing is characterized by a systemic and complex process of cellular and molecular activities. Dipotassium Glycyrrhizinate (DPG), a side product derived from glycyrrhizic acid, has several biological effects, such as being antiallergic, antioxidant, antibacterial, antiviral, gastroprotective, antitumoral, and anti-inflammatory. This study aimed to evaluate the anti-inflammatory effect of topical DPG on the healing of cutaneous wounds by secondary intention in an in vivo experimental model. Twenty-four male Wistar rats were used in the experiment, and were randomly divided into six groups of four. Circular excisions were performed and topically treated for 14 days after wound induction. Macroscopic and histopathological analyses were performed. Gene expression was evaluated by real-time qPCR. Our results showed that treatment with DPG caused a decrease in the inflammatory exudate as well as an absence of active hyperemia. Increases in granulation tissue, tissue reepithelization, and total collagen were also observed. Furthermore, DPG treatment reduced the expression of pro-inflammatory cytokines (Tnf-α, Cox-2, Il-8, Irak-2, Nf-kB, and Il-1) while increasing the expression of Il-10, demonstrating anti-inflammatory effects across all three treatment periods. Based on our results, we conclude that DPG attenuates the inflammatory process by promoting skin wound healing through the modulation of distinct mechanisms and signaling pathways, including anti-inflammatory ones. This involves modulation of the expression of pro- and anti-inflammatory cytokine expression; promotion of new granulation tissue; angiogenesis; and tissue re-epithelialization, all of which contribute to tissue remodeling.

Funder

CNPq

Coordination of Superior Level Staff Improvement (CAPES) scholarship

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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