Affiliation:
1. Guangdong Provincial Key Laboratory of Crop Genetic Improvement, South China Peanut Sub-Center of National Center of Oilseed Crops Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
2. Department of Plant Pathology, University of Georgia, Tifton, GA 30602, USA
3. Institute of Agricultural Sciences in Ganzhou, Ganzhou 341000, China
Abstract
Silicon (Si) has been shown to promote peanut growth and yield, but whether Si can enhance the resistance against peanut bacterial wilt (PBW) caused by Ralstonia solanacearum, identified as a soil-borne pathogen, is still unclear. A question regarding whether Si enhances the resistance of PBW is still unclear. Here, an in vitro R. solanacearum inoculation experiment was conducted to study the effects of Si application on the disease severity and phenotype of peanuts, as well as the microbial ecology of the rhizosphere. Results revealed that Si treatment significantly reduced the disease rate, with a decrement PBW severity of 37.50% as compared to non-Si treatment. The soil available Si (ASi) significantly increased by 13.62–44.87%, and catalase activity improved by 3.01–3.10%, which displayed obvious discrimination between non-Si and Si treatments. Furthermore, the rhizosphere soil bacterial community structures and metabolite profiles dramatically changed under Si treatment. Three significantly changed bacterial taxa were observed, which showed significant abundance under Si treatment, whereas the genus Ralstonia genus was significantly suppressed by Si. Similarly, nine differential metabolites were identified to involve into unsaturated fatty acids via a biosynthesis pathway. Significant correlations were also displayed between soil physiochemical properties and enzymes, the bacterial community, and the differential metabolites by pairwise comparisons. Overall, this study reports that Si application mediated the evolution of soil physicochemical properties, the bacterial community, and metabolite profiles in the soil rhizosphere, which significantly affects the colonization of the Ralstonia genus and provides a new theoretical basis for Si application in PBW prevention.
Funder
Guangdong Provincial Key Research and Development Program-Modern Seed Industry
China Agriculture Research System of MOF and MARA
National Natural Science Foundation of China
China Postdoctoral Science Foundation
Guangdong Basic and Applied Basic Research Foundation
Guangdong Provincial Department of Science and Technology Project-International Scientific and Technological Cooperation
Technology Special Fund of Guangdong Province Agriculture and Rural Affairs Department
Guangzhou Basic and Applied Basic Research Foundation
Agricultural Competitive Industry Discipline Team Building Project of Guangdong Academy of Agricultural Sciences
Special Fund for Scientific Innovation Strategy-Construction of High Level Academy of Agriculture Science
Foundation of Director of Crop Research Institute of Guangdong Academy of Agriculture Sciences
Open Fund of Guangdong Provincial Key Laboratory of Crop Genetic Improvement
Science and Technology Planning Project of Guangdong Province
Jiangxi Provincial Key R&D Program Project
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Cited by
2 articles.
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