Tumor Microenvironment Based on Extracellular Matrix Hydrogels for On-Chip Drug Screening

Author:

Liu Xiaoyan12ORCID,Cheng Jinxiong3,Zhao Yingcan4

Affiliation:

1. Institute for Health Innovation & Technology, National University of Singapore, Singapore 117599, Singapore

2. Department of Biomedical Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Road, Nanshan District, Shenzhen 518055, China

3. Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, 3700 O’Hara Street, Pittsburgh, PA 15260, USA

4. Environment Science Programme, Department of Life Sciences, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai 519087, China

Abstract

Recent advances in three-dimensional (3D) culturing and nanotechnology offer promising pathways to overcome the limitations of drug screening, particularly for tumors like neuroblastoma. In this study, we develop a high-throughput microfluidic chip that integrates a concentration gradient generator (CGG) with a 3D co-culture system, constructing the vascularized microenvironment in tumors by co-culturing neuroblastoma (SY5Y cell line) and human brain microvascular endothelial cells (HBMVECs) within a decellularized extracellular matrix (dECM) hydrogels. The automated platform enhances the simulation of the tumor microenvironment and allows for the precise control of the concentrations of nanomedicines, which is crucial for evaluating therapeutic efficacy. The findings demonstrate that the high-throughput platform can significantly accelerate drug discovery. It efficiently screens and analyzes drug interactions in a biologically relevant setting, potentially revolutionizing the drug screening process.

Funder

Zhuhai Basic and Applied Basic Research Foundation

UIC Start-up Research Fund

Guangdong University Key Research Project

Hong Kong HKSTP & HKBU Joint Innovation Fund

Publisher

MDPI AG

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