Genetic Variability in the E6/E7 Region of Human Papillomavirus 16 in Women from Ecuador-Reference-Cited by-同舟云学术

Genetic Variability in the E6/E7 Region of Human Papillomavirus 16 in Women from Ecuador

Published:2023-06-19 Issue:6 Volume:15 Page:1393
ISSN:1999-4915
Container-title:Viruses
language:en
Short-container-title:Viruses

Author:

Zhingre Alicia¹,Bedoya-Pilozo César²³,Gutiérrez-Pallo Diana⁴,Badano Inés⁵,Herrera-Yela Andrés⁶^ORCID,Salazar Zoila¹^ORCID,Alarcón Damaris⁴^ORCID,Argüello-Bravo Natali⁷,Espinoza Maylen⁸,Ponce Patricio⁹,Soto Yudira¹⁰,Carrazco-Montalvo Andrés⁴¹¹^ORCID

Affiliation:

1. Laboratorio de Biotecnología, Universidad Católica de Cuenca, Cuenca 010107, Ecuador

2. Hospital Luis Vernaza, Guayaquil 090306, Ecuador

3. Lab Genetics Ballenita, Santa Elena 241701, Ecuador

4. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”, Centro de Referencia Nacional de Genómica, Secuenciación y Bioinformática, Quito 170403, Ecuador

5. Laboratorio de Antropología Biológica y Bioinformática Aplicada, Red de Laboratorios, Universidad Nacional de Misiones and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Posadas N3300, Argentina

6. Facultad de Ingeniería, Maestría en Biología Computacional, Pontificia Universidad Católica del Ecuador, Quito 170803, Ecuador

7. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”, Laboratorio de Influenza y Otros Virus Respiratorios, Quito 170403, Ecuador

8. Hospital IESS CEIBOS, Guayaquil 090615, Ecuador

9. Instituto Nacional de Investigación en Salud Pública “Leopoldo Izquieta Pérez”, Gestión de Investigación, Desarrollo e Innovación, Quito 170403, Ecuador

10. Instituto de Medicina Tropical “Pedro Kouri”, Laboratorio de Infecciones de Transmisión Sexual, Departamento de Virología, Autopista Novia del Mediodía Km 6 ½, La Habana 10100, Cuba

11. Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, 28049 Madrid, Spain

Abstract

Human Papillomavirus (HPV) infection is associated with intraepithelial neoplasia and cervical cancer (CC). Ecuador has a high prevalence of cervical cancer, with more than 1600 new cases diagnosed annually. This study aimed to analyze oncogenes E6 and E7 of HPV16 in samples collected from women with cancerous and precancerous cervical lesions from the Ecuadorian coast. Twenty-nine women, including six with ASCUS, three with LSIL, thirteen with HSIL, and seven with Cacu, were analyzed. The most common SNPs were E6 350G or L83V (82.6%) and E6 145T/286A/289G/335T/350G or Q14H/F78Y/L83V (17.4%). Both variants are reported to be associated with an increased risk of cervical cancer in worldwide studies. In contrast, all E7 genes have conserved amino-acid positions. Phylogenetic trees showed the circulation of the D (26.1%) and A (73.9) lineages. The frequency of D was higher than that reported in other comparable studies in Ecuador and Latin America, and may be related to the ethnic composition of the studied populations. This study contributes to the characterization of the potential risk factors for cervical carcinogenesis associated with Ecuadorian women infected with HPV16.

Funder

SENESCYT

Publisher

MDPI AG

Subject

Virology,Infectious Diseases

Link

https://www.mdpi.com/1999-4915/15/6/1393/pdf

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