Formulation and Characterization of Ethyl Cellulose-Based Patches Containing Curcumin-Chitosan Nanoparticles for the Possible Management of Inflammation via Skin Delivery
Author:
Nawaz Asif1ORCID, Latif Muhammad Shahid1ORCID, Shah Muhammad Khurshid Alam1, Elsayed Tarek M.2ORCID, Ahmad Saeed3ORCID, Khan Hamid Ali4
Affiliation:
1. Advanced Drug Delivery Lab, Gomal Centre of Pharmaceutical Sciences, Faculty of Pharmacy, Gomal University, Dera Ismail Khan 29050, Pakistan 2. Pharmaceutical Technology Department, Faculty of Pharmacy, Sultan Zainal Abidin University, Besut Kampus, Besut 22200, Malaysia 3. Institute of Biotechnology and Microbiology, Bacha Khan University, Charsadda 24420, Pakistan 4. Directorate of ORIC, Bacha Khan University, Charsadda 24420, Pakistan
Abstract
Curcumin, a natural phenolic compound, exhibits poor absorption and extensive first pass metabolism after oral administration. In the present study, curcumin-chitosan nanoparticles (cur-cs-np) were prepared and incorporated into ethyl cellulose patches for the management of inflammation via skin delivery. Ionic gelation method was used for the preparation of nanoparticles. The prepared nanoparticles were evaluated for size, zetapotential, surface morphology, drug content, and % encapsulation efficiency. The nanoparticles were then incorporated into ethyl cellulose-based patches using solvent evaporation technique. ATR-FTIR was used to study/assess incompatibility between drug and excipients. The prepared patches were evaluated physiochemically. The in vitro release, ex vivo permeation, and skin drug retention studies were carried out using Franz diffusion cells and rat skin as permeable membrane. The prepared nanoparticles were spherical, with particle size in the range of 203–229 nm, zetapotential 25–36 mV, and PDI 0.27–0.29 Mw/Mn. The drug content and %EE were 53% and 59%. Nanoparticles incorporated patches are smooth, flexible, and homogenous. The in vitro release and ex vivo permeation of curcumin from nanoparticles were higher than the patches, whereas the skin retention of curcumin was significantly higher in case of patches. The developed patches deliver cur-cs-np into the skin, where nanoparticles interact with skin negative charges and hence result in higher and prolonged retention in the skin. The higher concentration of drug in the skin helps in better management of inflammation. This was shown by anti-inflammatory activity. The inflammation (volume of paw) was significantly reduced when using patches as compared to nanoparticles. It was concluded that the incorporation of cur-cs-np into ethyl cellulose-based patches results in controlled release and hence enhanced anti-inflammatory activity.
Funder
Higher Education Commission of Pakistan
Subject
Polymers and Plastics,Organic Chemistry,Biomaterials,Bioengineering
Reference33 articles.
1. Multi-antibacterial agent-based electrospun polycaprolactone for active wound dressing;Safdari;Prog. Biomater.,2022 2. Witika, B.A., Makoni, P.A., Matafwali, S.K., Mweetwa, L.L., Shandele, G.C., and Walker, R.B. (2021). Enhancement of biological and pharmacological properties of an encapsulated polyphenol: Curcumin. Molecules, 26. 3. Md, S., Mujtaba, M.A., Lim, W.M., Alhakamy, N.A., Zeeshan, F., and Hosny, K.M. (2021). Nanoemulsions in Food Technology, CRC Press. 4. Porro, C., Cianciulli, A., Trotta, T., Lofrumento, D.D., and Panaro, M.A. (2019). Curcumin regulates anti-inflammatory responses by JAK/STAT/SOCS signaling pathway in BV-2 microglial cells. Biology, 8. 5. Curcumin: A modulator of inflammatory signaling pathways in the immune system;Kahkhaie;Inflammopharmacology,2019
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