Affiliation:
1. The Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations, Chongqing Normal University, Chongqing 401331, China
2. Chongqing Engineering Research Center of Specialty Crop Resources, The College of Life Science, Chongqing Normal University, Chongqing 401331, China
Abstract
Verticillium dahliae, a virulent soil-borne fungus, elicits Verticillium wilt in numerous dicotyledonous plants through intricate pathogenic mechanisms. Ubiquitination, an evolutionarily conserved post-translational modification, marks and labels proteins for degradation, thereby maintaining cellular homeostasis. Within the ubiquitination cascade, ubiquitin ligase E3 demonstrates a unique capability for target protein recognition, a function often implicated in phytopathogenic virulence. Our research indicates that two ubiquitin ligase E3s, VdBre1 and VdHrd1, are intrinsically associated with virulence. Our findings demonstrate that the deletion of these two genes significantly impairs the ability of V. dahliae to colonize the vascular bundles of plants and to form typical penetration pegs. Furthermore, transcriptomic analysis suggests that VdBre1 governs the lipid metabolism pathway, while VdHrd1 participates in endoplasmic-reticulum-related processes. Western blot analyses reveal a significant decrease in histone ubiquitination and histone H3K4 trimethylation levels in the ΔVdBre1 mutant. This research illuminates the function of ubiquitin ligase E3 in V. dahliae and offers fresh theoretical perspectives. Our research identifies two novel virulence-related genes and partially explicates their roles in virulence-associated structures and gene regulatory pathways. These findings augment our understanding of the molecular mechanisms inherent to V. dahliae.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Chongqing
Subject
Plant Science,Ecology, Evolution, Behavior and Systematics,Microbiology (medical)
Reference45 articles.
1. A patent review of the ubiquitin ligase system: 2015-2018;Li;Expert. Opin. Ther. Pat.,2018
2. The Ubiquitin-Proteasome System and F-box Proteins in Pathogenic Fungi;Liu;Mycobiology,2011
3. Chen, Q., Li, Y., Wang, J., Li, R., and Chen, B. (2018). cpubi4 Is Essential for Development and Virulence in Chestnut Blight Fungus. Front. Microbiol., 9.
4. The lore of the RINGs: Substrate recognition and catalysis by ubiquitin ligases;Jackson;Trends Cell Biol.,2000
5. The HECT family of E3 ubiquitin ligases: Multiple players in cancer development;Bernassola;Cancer Cell,2008