Abstract
The study aimed to evaluate cryo-injury during the cryopreservation in Sorubim cuspicaudus sperm with ethylene glycol (EG) at different rates (6, 8, 10%). Fresh, prefrozen, and post-thawed sperm quality as motility total, velocities, mitochondria damage (Mit-d), membrane damage (Mem-d), and DNA fragmentation (DNA-f), were examined. The Mit-d, Mem-d, and DNA-f were evaluated through flow cytometry. High motility (>95%) and a low percentage of Mem-d (1.0 ± 0.5%), Mit-d (1.4 ± 0.9%), and DNA-f (2.4 ± 0.8%) were recorded for fresh semen. Prefrozen semen increases in Mit-d and DNA-f were observed compared to fresh semen (p < 0.05). In thawed semen, increased Mit-d (2.6 to 3-fold), Mem-d (6 to 1-fold), and DNA-f (3.3 to 6.6-fold) compared to prefrozen was observed. Thawed semen showed Mit-d (34 to 37-fold), Mem-d (24.5 to 26.6-fold) and DNA-f (13 to 18.5-fold) increased high. In conclusion, the present study demonstrated that mitochondria, membrane, and DNA integrity undergo significant damage during both pre-freezing and freezing/thawing with EG inclusion percentages from 6 to 10% that affect its fertilizing capacity, which is reduced to half of that obtained with fresh semen. It is suggested that a cryoprotective solution composed of 6% EG, 6% glucose, and 5% skimmed milk powder is a useful protocol for the cryopreservation of S. cuspicaudus semen.
Funder
Empresas Públicas de Medellín (EPM) and the University of Córdoba
Subject
General Veterinary,Animal Science and Zoology
Reference54 articles.
1. Sorubim cuspicaudus, a new long whiskered catfish from northwestern South America (Siluriformes: Pimelodidae);Littmann;Proc. Biol. Soc. Wash.,2000
2. Mojica, J.I., Usma, S., Álvarez-León, R., and Lasso, C. (2012). Libro Rojo de Peces Dulceacuícolas de Colombia 2012, Universidad de Manizales.
3. Crioconservación de semen de blanquillo Sorubim cuspicaudus con dimetilacetamida como crioprotector;Rev. CES Med. Vet.,2015
4. Evaluación de etilenglicol como crioprotector en la crioconservación de semen de bagre blanco (Sorubim cuspicaudus, Pimelodidae);Dorado;Acta Biol. Colom.,2014
5. Factors enhancing fish sperm quality and emerging tools for sperm analysis;Cabrita;Aquaculture,2014
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献