Human Dental Pulp Mesenchymal Stem Cell-Derived Soluble Factors Combined with a Nanostructured Scaffold Support the Generation of a Vascular Network In Vivo
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Published:2023-09-02
Issue:17
Volume:13
Page:2479
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ISSN:2079-4991
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Container-title:Nanomaterials
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language:en
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Short-container-title:Nanomaterials
Author:
Barone Ludovica1ORCID, Gallazzi Matteo2, Rossi Federica1ORCID, Papait Roberto1, Raspanti Mario3ORCID, Zecca Piero Antonio3ORCID, Buonarrivo Luca1, Bassani Barbara2, Bernardini Giovanni1ORCID, Bruno Antonino12ORCID, Gornati Rosalba1ORCID
Affiliation:
1. Department of Biotechnology and Life Sciences, University of Insubria, 21100 Varese, Italy 2. Laboratory of Innate Immunity, Unit of Molecular Pathology, Biochemistry, and Immunology, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) MultiMedica, 20138 Milan, Italy 3. Department of Medicine and Innovative Technology, University of Insubria, 21100 Varese, Italy
Abstract
Among all strategies directed at developing new tools to support re-vascularization of damaged tissues, the use of pro-angiogenic soluble factors, derived from mesenchymal stem cells (MSCs), appears a promising approach for regenerative medicine. Here, we compared the feasibility of two devices, generated by coupling soluble factors of human dental pulp mesenchymal stem cells (DPSCs), with a nanostructured scaffold, to support angiogenesis once transplanted in mice. DPSCs were obtained from impacted wisdom tooth removal, usually considered surgical waste material. After 28 days, we verified the presence of active blood vessels inside the scaffold through optical and scansion electron microscopy. The mRNA expression of surface antigens related to macrophage polarization (CD68, CD80, CD86, CD163, CD206), as well as pro-angiogenic markers (CD31, CD34, CD105, Angpt1, Angpt2, CDH5) was evaluated by real-time PCR. Our results demonstrate the capability of DPSC–scaffold and DPSC soluble factors–scaffold to support angiogenesis, similarly to adipose stem cells, whereas the absence of blood vessels was found in the scaffold grafted alone. Our results provide evidence that DPSC-conditioned medium can be proposed as a cell-free preparation able to support angiogenesis, thus, providing a relevant tool to overcome the issues and restrictions associated with the use of cells.
Funder
Fondo Comune di Ateneo per la Ricerca–University of Insubria, Italy Italian Association for Cancer Research Ricerca Corrente Rete Cardiologica IRCCS 2022 Ricerca Corrente, IRCCS MultiMedica Life Sciences and Biotechnology at the University of Insubria, Varese Italian Ministry of University and Research PRIN 2017
Subject
General Materials Science,General Chemical Engineering
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