Neuroprotective Effects of Water Extract from Brown Algae Petalonia binghamiae in an Experimental Model of Focal Cerebral Ischemia In Vitro and In Vivo

Author:

Eom Sun Ho1,Hong Geum-Lan2ORCID,Kang Hyun Bae1,Lee Nam-Seob2,Kim Do Kyung2ORCID,Jeong Young Gil2,Kim Chun-Sung3,Yoo Yung Choon4,Lee Bong Ho5,Jung Ju-Young6ORCID,Kim Dong-Sub7ORCID,Han Seung Yun2ORCID

Affiliation:

1. Healinnols Inc., Daejeon 34054, Republic of Korea

2. Department of Anatomy, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea

3. Department of Oral Biochemistry, College of Dentistry, Chosun University, Gwangju 61452, Republic of Korea

4. Department of Microbiology, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea

5. Department of Chemical Technology, Hanbat National University, Daejeon 34158, Republic of Korea

6. Department of Histology & Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea

7. Division of Natural Product Research, Korea Prime Pharmacy Co., Ltd., Gwangju 61473, Republic of Korea

Abstract

Focal cerebral ischemia (fCI) can result in brain injury and sensorimotor deficits. Brown algae are currently garnering scientific attention as potential therapeutic candidates for fCI. This study investigated the therapeutic effects of the hot water extract of Petalonia binghamiae (wPB), a brown alga, in in vitro and in vivo models of fCI. The neuroprotective efficacy of wPB was evaluated in an in vitro excitotoxicity model established using HT-22 cells challenged with glutamate. Afterward, C57/BL6 mice were administered wPB for 7 days (10 or 100 mg/kg, intragastric) and subjected to middle cerebral artery occlusion and reperfusion (MCAO/R) operation, which was used as an in vivo fCI model. wPB co-incubation significantly inhibited cell death, oxidative stress, and apoptosis, as well as stimulated the expression of heme oxygenase-1 (HO-1), an antioxidant enzyme, and the nuclear translocation of its upstream regulator, nuclear factor erythroid 2-related factor 2 (Nrf2) in HT-22 cells challenged with glutamate-induced excitotoxicity. Pretreatment with either dose of wPB significantly attenuated infarction volume, neuronal death, and sensorimotor deficits in an in vivo fCI model. Furthermore, the attenuation of oxidative stress and apoptosis in the ischemic lesion accompanied the wPB-associated protection. This study suggests that wPB can counteract fCI via an antioxidative effect, upregulating the Nrf2/HO-1 pathway.

Funder

Ministry of Oceans and Fisheries, Korea

National Research Foundation, Korea

Publisher

MDPI AG

Subject

Microbiology (medical),Molecular Biology,General Medicine,Microbiology

Reference54 articles.

1. Gender and stroke symptoms: A review of the current literature;Beal;J. Neurosci. Nurs.,2010

2. Deaths: Leading causes for 2004;Heron;Natl. Vital. Stat. Rep.,2007

3. Beyond NMDA and AMPA glutamate receptors: Emerging mechanisms for ionic imbalance and cell death in stroke;Besancon;Trends Pharmacol. Sci.,2008

4. Ramon, R., and Julio César, G. (2016). Ischemic Stroke, IntechOpen.

5. Selective dysfunction of hippocampal CA1 astrocytes contributes to delayed neuronal damage after transient forebrain ischemia;Ouyang;J. Neurosci.,2007

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3