In Vivo Toxicity Assessment of the Probiotic Bacillus amyloliquefaciens HTI-19 Isolated from Stingless Bee (Heterotrigona itama) Honey-Reference-Cited by-同舟云学术

In Vivo Toxicity Assessment of the Probiotic Bacillus amyloliquefaciens HTI-19 Isolated from Stingless Bee (Heterotrigona itama) Honey

Published:2023-05-19 Issue:10 Volume:15 Page:2390
ISSN:2072-6643
Container-title:Nutrients
language:en
Short-container-title:Nutrients

Author:

Zulkhairi Amin Fatin Aina¹,Shafiq Cheng Mohamad Zulhafiz¹,Sabri Suriana²³^ORCID,Ismail Norsharina¹^ORCID,Chan Kim Wei¹^ORCID,Mohd Esa Norhaizan¹⁴^ORCID,Mohd Lila Mohd Azmi⁵,Nur-Fazila Saulol Hamid⁵,Khalifa Shaden A. M.⁶,El-Seedi Hesham R.⁷⁸,Zawawi Norhasnida⁹¹⁰^ORCID

Affiliation:

1. Natural Medicines and Product Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia

2. Enzyme and Microbial Technology Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia

3. Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia

4. Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia

5. Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia

6. Department of Molecular Biosciences, Wenner-Gren Institute, Stockholm University, SE-106 91 Stockholm, Sweden

7. International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang 212013, China

8. International Joint Research Laboratory of Intelligent Agriculture and Agri-Products Processing, Jiangsu Education Department, Jiangsu University, Zhenjiang 212013, China

9. Functional Carbohydrate and Protein Laboratory, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia

10. Laboratory of Halal Science, Halal Products Research Institute, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia

Abstract

This study evaluated the acute and sub-acute toxicity of B. amyloliquefaciens HTI-19 (isolated from stingless bee honey) in female Sprague Dawley rats. In an acute toxicity study, the rats received a low dosage (1 × 109 CFU·mL−1), medium dosage (3 × 109 CFU·mL−1), or high dosage (1 × 1010 CFU·mL−1) of B. amyloliquefaciens HTI-19 daily orally by syringe-feeding for 14 days. For the subacute toxicity study, rats received a low dosage (1 × 109 CFU·mL−1) or a high dosage (1 × 1010 CFU·mL−1) for 28 days. The probiotic feeding in acute and sub-acute toxicity studies showed no mortality or significant abnormalities in rats throughout the experimental period. In week 2 of the acute study, the body weight of the rats showed a significant increase (p < 0.05) compared to the control. By gross and microscopic examination of organs, no evidently significant changes were observed in the morphology of organs. Serum biochemical tests and blood hematology tests also revealed no treatment-related changes. Overall, these data indicated that oral administration of B. amyloliquefaciens HTI-19 up to 1 × 109 CFU·mL−1 for 28 days can be considered safe.

Funder

Ministry of Education

Graduate Research Fellowship

Publisher

MDPI AG

Subject

Food Science,Nutrition and Dietetics

Link

https://www.mdpi.com/2072-6643/15/10/2390/pdf

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