Specific Monoclonal Antibodies against African Swine Fever Virus Protease pS273R Revealed a Novel and Conserved Antigenic Epitope

Author:

Zhang Jiajia1234,Zhang Kaili1234,Sun Shaohua1234,He Ping1234,Deng Dafu1234,Zhang Pingping1234,Zheng Wanglong1234ORCID,Chen Nanhua1234ORCID,Zhu Jianzhong1234ORCID

Affiliation:

1. College Veterinary Medicine, Yangzhou University, Yangzhou 225009, China

2. Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University, Yangzhou 225009, China

3. Comparative Medicine Research Institute, Yangzhou University, Yangzhou 225009, China

4. Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China

Abstract

The African swine fever virus (ASFV) is a large enveloped DNA virus that causes a highly pathogenic hemorrhagic disease in both domestic pigs and wild boars. The ASFV genome contains a double-stranded DNA encoding more than 150 proteins. The ASFV possesses only one protease, pS273R, which is important for virion assembly and host immune evasion. Therefore, the specific monoclonal antibody (mAb) against pS273R is useful for ASFV research. Here, we generated two specific anti-pS273R mAbs named 2F3 and 3C2, both of which were successfully applied for ELISA, Western blotting, and immunofluorescence assays. Further, we showed that both 2F3 and 3C2 mAbs recognize a new epitope of N terminal 1–25 amino acids of pS273R protein, which is highly conserved across different ASFV strains including all genotype I and II strains. Based on the recognized epitope, an indirect ELISA was established and was effective in detecting antibodies during ASFV infection. To conclude, the specific pS273R mAbs and corresponding epitope identified will strongly promote ASFV serological diagnosis and vaccine research.

Funder

National Key Research and Development Program of China

Jiangsu Provincial Key R&D Plan

Jiangsu Agricultural Science and Technology Independent Innovation Fund project

111 Project

Priority Academic Program Development of Jiangsu Higher Education Institutions

Research and Practice Innovation Project of Jiangsu Province Graduate Students

Publisher

MDPI AG

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