Expression of Growth Hormone-Releasing Hormone and Its Receptor Splice Variants in a Cohort of Hungarian Pediatric Patients with Hematological and Oncological Disorders: A Pilot Study

Author:

Juhász Éva1,Szabó Zsuzsanna2,Schally Andrew V.3456,Király József2,Fodor Petra2,Kónya Gábor2,Dezső Balázs7,Szabó Erzsébet8,Halmos Gábor23,Kiss Csongor1ORCID

Affiliation:

1. Department of Pediatrics, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary

2. Department of Biopharmacy, Faculty of Pharmacy, University of Debrecen, 4032 Debrecen, Hungary

3. Veterans Affairs Medical Center, Endocrine, Polypeptide and Cancer Institute, Miami, FL 33125, USA

4. Department of Pathology, Miller School of Medicine, University of Miami, Miami, FL 33101, USA

5. Department of Medicine, Divisions of Hematology-Oncology and Endocrinology, Miller School of Medicine, University of Miami, Miami, FL 33101, USA

6. Sylvester Comprehensive Cancer Center, University of Miami, Miami, FL 33136, USA

7. Department of Pathology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary

8. Department of Pharmacology, Faculty of Pharmacy, HUN-REN-DE Pharmamodul Research Group, University of Debrecen, Nagyerdei krt. 98, 4032 Debrecen, Hungary

Abstract

Hematological and oncological diseases are still among the leading causes of childhood mortality. Expression of growth hormone-releasing hormone (GHRH) and its receptors (GHRH-R) has been previously demonstrated in various human tumors, but very limited findings are available about the presence and potential function of GHRH-Rs in oncological and hematological disorders of children. In this study, we aimed to investigate the expression of mRNA for GHRH and splice variant 1 (SV) of GHRH-R in 15 pediatric hematological/oncological specimens by RT-PCR. The presence and binding characteristics of GHRH-R protein were also studied by Western blot and ligand competition assays. Of the fifteen specimens studied, eleven pediatric samples (73%) showed the expression of mRNA for GHRH. These eleven samples also expressed mRNA for GHRH receptor SV1. GHRH-R protein was found to be expressed in two benign tumor samples and five malignant tumors examined by Western blot. The presence of specific, high affinity binding sites on GHRH-R was demonstrated in all of the seven human pediatric solid tumor samples investigated. Our results show that the expression of GHRH and SV1 of GHRH-R in hemato-oncological diseases in children can pave the way for further investigation of GHRH-Rs as potential molecular targets for diagnosis and therapy.

Funder

Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund

National Research, Development and Innovation Fund and the Foundation for Children with Leukemia

Publisher

MDPI AG

Reference36 articles.

1. Antagonists of growth-hormone-releasing hormone: An emerging new therapy for cancer. Nature clinical practice;Schally;Endocrinol. Metab.,2008

2. Molecular cloning and expression of a pituitary-specific receptor for growth hormone-releasing hormone;Mayo;Mol. Endocrinol.,1992

3. Growth Hormone Releasing Hormone (GHRH) and the GHRH Receptor;Wajnrajch;Rev. Endocr. Metab. Disord.,2002

4. Hypothalamic hormones and cancer;Schally;Front. Neuroendocrinol.,2001

5. Vélez, E.J., and Unniappan, S. (2020). A Comparative Update on the Neuroendocrine Regulation of Growth Hormone in Vertebrates. Front. Endocrinol., 11.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3