Genome Identification and Evolutionary Analysis of LBD Genes and Response to Environmental Factors in Phoebe bournei
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Published:2023-08-09
Issue:16
Volume:24
Page:12581
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Ma Yiming1, Zhong Minchen2, Li Jingshu2, Jiang Yiming3, Zhou Xuerong4ORCID, Justice Ijeoma Chris1, Tang Xinghao5, Chen Shipin2, Cao Shijiang2
Affiliation:
1. International College, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China 3. Horticultrue College, Fujian Agriculture and Forestry University, Fuzhou 350002, China 4. Commonwealth Scientific Industrial Research Organization (CSIRO) Agriculture Food, Canberra, ACT 2601, Australia 5. Fujian Academy of Forestry, Fuzhou 350002, China
Abstract
Phoebe bournei is nationally conserved in China due to its high economic value and positive effect on the ecological environment. P. bournei has an excellent wood structure, making it useful for industrial and domestic applications. Despite its importance, there are only a few studies on the lateral organ boundary domain (LBD) genes in P. bournei. The LBD gene family contributes to prompting rooting in multiple plant species and therefore supports their survival directly. To understand the LBD family in P. bournei, we verified its characteristics in this article. By comparing the sequences of Arabidopsis and identifying conserved domains and motifs, we found that there were 38 members of the LBD family in P. bournei, which were named PbLBD1 to PbLBD38. Through evolutionary analysis, we found that they were divided into two different populations and five subfamilies in total. The LBD gene family in P. bournei (Hemsl.) Yang species had two subfamilies, including 32 genes in Class I and 6 genes in Class II. It mainly consists of a Lateral Organ Boundary (LOB) conservative domain, and the protein structure is mostly “Y”-shaped. The gene expression pattern of the LBD gene family showed that the LBD genes were mainly expressed in lateral organs of plants, such as flowers and fruits. The response of LBD transcription factors to red and blue light was summarized, and several models of optogenetic expression regulation were proposed. The effect of regulatory mechanisms on plant rooting was also predicted. Moreover, quantitative real-time PCR (qRT-PCR) revealed that most PbLBDs were differentially expressed under cold, heat, drought, and salt stresses, indicating that PbLBDs might play different functions depending on the type of abiotic stress. This study provides the foundation for further research on the function of LBD in this tree species in the future.
Funder
the Project of Forestry Peak Discipline at Fujian Agriculture and Forestry University, China the 7th Project of Forest Seeding Breaking in Fujian Province Fujian Forestry seedling science and technology project, construction plan of Peak discipline of Fujian Agriculture and Forestry University Fujian Province Seed Industry Innovation and Industrialization Project “Innovation and Industrialization Development of Precious Tree Seed Industries (P. bournei)” the sub-project of the National Key R&D Program “P. bournei Efficient Cultivation Technology”
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
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