Affiliation:
1. Biochemical Neuropharmacology Laboratory, Kazan Federal University, 18 ul. Kremlevskaya, 420008 Kazan, Russia
Abstract
Partial reversible inhibition of enzymes, also called hyperbolic inhibition, is an uncommon mechanism of reversible inhibition, resulting from a productive enzyme–inhibitor complex. This type of inhibition can involve competitive, mixed, non-competitive and uncompetitive inhibitors. While full reversible inhibitors show linear plots for reciprocal enzyme initial velocity versus inhibitor concentration, partial inhibitors produce hyperbolic plots. Similarly, dose–response curves show residual fractional activity of enzymes at high doses. This article reviews the theory and methods of analysis and discusses the significance of this type of reversible enzyme inhibition in metabolic processes, and its implications in pharmacology and toxicology.
Funder
Strategic Academic Leadership Program “Priority 2030” of the Kazan Federal University of the Government of the Russian Federation
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference42 articles.
1. The Kinetics of Enzyme-Catalyzed Reactions with Two or More Substrates or Products;Cleland;Biochim. Biophys. Acta BBA—Spec. Sect. Enzymol. Subj.,1963
2. An Allosteric Reduced Nicotinamide Adenine Dinucleotide Oxidase from Mycobacterium Tuberculosis;Worcel;J. Biol. Chem.,1965
3. Effect of Glucose 6-Phosphate on the Nucleotide Site of Glycogen Phosphorylase b;Wang;J. Biol. Chem.,1970
4. Segel, I.H. (1975). Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady State Enzyme Systems, Wiley.
5. A Graphical Method for Determining Inhibition Parameters for Partial and Complete Inhibitors;Yoshino;Biochem. J.,1987