Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses

Author:

Park Jihyun1,Nguyen Thi Minh Nguyet1,Park Hyun-ah1,Nguyen My Tuyen Thi23,Lee Nan-young1,Ban So-young12,Park Kyu-been1,Lee Chang-kyu1,Kim Jaehan2ORCID,Park Jong-Tae14ORCID

Affiliation:

1. CARBOEXPERT Inc., Daejeon 34134, Republic of Korea

2. Department of Food and Nutrition, Chungnam National University, Daejeon 34134, Republic of Korea

3. College of Agriculture, Can Tho University, Can Tho City 94115, Vietnam

4. Department of Food Science and Technology, Chungnam National University, Daejeon 34134, Republic of Korea

Abstract

Inotodiol, a lanostane-type triterpenoid, and many phytochemicals from Chaga mushrooms have been investigated for various allergic diseases. However, the anti-aging and anti-inflammatory activities of inotodiol under different types of oxidative stress and the impact of inotodiol on collagen and hyaluronan synthesis have not been sufficiently studied. Lanostane triterpenoids-rich concentrate, which contained 10% inotodiol as major (inotodiol concentrate), was prepared from Chaga and compared with pure inotodiol in terms of anti-inflammatory activities on a human keratinocyte cell line, HaCaT cells, under various stimulations such as stimulation with ultraviolet (UV) B or tumor necrosis factor (TNF)-α. In stimulation with TNF-α, interleukin (IL)-1β, IL-6, and IL-8 genes were significantly repressed by 0.44~4.0 μg/mL of pure inotodiol. UVB irradiation induced the overexpression of pro-inflammatory cytokines, but those genes were significantly suppressed by pure inotodiol or inotodiol concentrate. Moreover, pure inotodiol/inotodiol concentrate could also modulate the synthesis of collagen and hyaluronic acid by controlling COL1A2 and HAS2/3 expression, which implies a crucial role for pure inotodiol/inotodiol concentrate in the prevention of skin aging. These results illuminate the anti-inflammatory and anti-aging effects of pure inotodiol/inotodiol concentrate, and it is highly conceivable that pure inotodiol and inotodiol concentrate could be promising natural bioactive substances to be incorporated in therapeutic and beautifying applications.

Funder

Technology Commercialization support program, through the Korea Innovation Foundation funded by the Ministry of Science and ICT

Ministry of SMEs and Startups

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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