Immunomodulatory Effects Mediated by Nano Amorphous Calcium Phosphate/Chitosan Oligosaccharide Lactate Coatings Decorated with Selenium on Titanium Implants

Author:

Pantović Pavlović Marijana R.12,Ignjatović Nenad L.3ORCID,Panić Vladimir V.124ORCID,Mirkov Ivana I.5,Kulaš Jelena B.5,Malešević Anastasija Lj.5,Pavlović Miroslav M.12

Affiliation:

1. Department of Electrochemistry, Institute of Chemistry, Technology and Metallurgy, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia

2. Center of Excellence in Chemistry and Environmental Engineering—ICTM, University of Belgrade, 11000 Belgrade, Serbia

3. Institute of Technical Science of the Serbian Academy of Sciences and Arts, 11000 Belgrade, Serbia

4. Department of Chemical-Technological Sciences, State University of Novi Pazar, 36300 Novi Pazar, Serbia

5. Immunotoxicology Group, Department of Ecology, Institute for Biological Research “Sinisa Stankovic”—National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia

Abstract

The aim of this work is in situ anodization/anaphoretic deposition of a nano amorphous calcium phosphate (ACP)/chitosan oligosaccharide lactate (ChOL) multifunctional hybrid coating decorated with selenium (Se) on a titanium substrate and in vivo investigation of its immunomodulatory and anti-inflammatory effect. Investigating phenomena at the implant–tissue interface of interest for controlled inflammation and immunomodulation was also the aim of the research. In our earlier research, we designed coatings based on ACP and ChOL on titanium with anticorrosive, antibacterial and biocompatible properties, while in the presented results we show that selenium addition makes this coating an immunomodulator. The immunomodulatory effect of the novel hybrid coating is characterized by the examination of the functional aspects in the tissue around the implant (in vivo): proinflammatory cytokines’ gene expression, M1 (iNOS) and M2 (Arg1) macrophages, fibrous capsule formation (TGF-β) and vascularization (VEGF). The EDS, FTIR and XRD analyses prove the formation of a ACP/ChOL/Se multifunctional hybrid coating on Ti and the presence of Se. A higher M2/M1 macrophage ratio in the ACP/ChOL/Se-coated implants compared to pure titanium implants (a higher level of Arg1 expression) is noted at all time points examined (after 7, 14 and 28 days). Lower inflammation measured by gene expression of proinflammatory cytokines IL-1β and TNF, lower expression of TGF-β in the surrounding tissue and higher IL-6 expression (solely at day 7 post-implantation) is noted in presence of the ACP/ChOL/Se-coated implants.

Funder

Ministry of Science, Technological Development and Innovation of the Republic of Serbia

Publisher

MDPI AG

Subject

Biomedical Engineering,Biomaterials

Reference54 articles.

1. A State-of-the-Art Review of the Fabrication and Characteristics of Titanium and Its Alloys for Biomedical Applications;Sarraf;Bio-Des. Manuf.,2022

2. Titanium Alloys for Biomedical Applications;Rack;Mater. Sci. Eng. C,2006

3. Hydroxyapatite-Coated Titanium for Orthopedic Implant Applications;Cook;Clin. Orthop. Relat. Res.,1988

4. Mucalo, M.B.T. (2015). Woodhead Publishing Series in Biomaterials, Woodhead Publishing.

5. Nanosized and Nanocrystalline Calcium Orthophosphates;Dorozhkin;Acta Biomater.,2010

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