First Molecular Evidence of Seewis Virus in Croatia

Author:

Svoboda Karić Petra1,Anđelić Dmitrović Barbara1,Mrmić Stella1,Paić Antonia1,Bjedov Linda2,Štritof Zrinka3ORCID,Margaletić Josip2,Kurolt Ivan-Christian1ORCID

Affiliation:

1. Research Department, University Hospital for Infectious Diseases “Dr. Fran Mihaljević”, Mirogojska 8, 10000 Zagreb, Croatia

2. Department of Forest Protection and Wildlife Management, Faculty of Forestry, University of Zagreb, Svetošimunska 25, 10000 Zagreb, Croatia

3. Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia

Abstract

Orthohantaviruses are mainly carried and transmitted by wild rodents, although during the last decade, they have also been identified in multiple species of shrews and moles. Orthohantavirus, Orthohantavirus seewisense (Seewis virus, SWSV), first detected in Switzerland in a single Sorex araneus (Eurasian common shrew) specimen, has been further described in several European countries, including Croatia’s neighboring Slovenia and Hungary. Croatia is a well-known endemic region for several zoonotic agents including three different orthohantaviruses: Orthohantavirus puumalaense (PUUV), Orthohantavirus dobravaense (DOBV), and Orthohantavirus tulaense (TULV). In this study, nine shrews were tested and SWSV RNA was detected in liver, lung, and kidney belonging to two shrews (22.22%), one collected on Medvednica mountain in Zagreb County, and the other in the Stara Gradiška area in lowland Croatia. The phylogenetic analysis of the complete S segment’s open reading frame (ORF) and partial L-segment revealed that the Croatian sequences, when compared to sequences from the adjacent geographic regions, form a specific genetic lineage. Two SWSV-positive shrew species—Sorex araneus and Neomys milleri (Mediterranean water shrew)—were identified using barcode-based sequence analysis. Therefore, the SWSV detection in N. milleri throughout the course of this study is seen as a rare find in this shrew species. To our knowledge, this is the first molecular and phylogenetic analysis of SWSV in Croatia.

Funder

Croatian Science Foundation

Strengthening the capacity of CerVirVac for research in virus immunology and vaccinology

Scientific Centre of Excellence for Virus Immunology and Vaccines

European Regional Development Fund

Croatian Forests Ltd. Zagreb

Zagreb City Office for Emergency Management

Publisher

MDPI AG

Subject

Paleontology,Space and Planetary Science,General Biochemistry, Genetics and Molecular Biology,Ecology, Evolution, Behavior and Systematics

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