The Oxidative Phosphorylation and Cytoskeleton Proteins of Mouse Ovaries after 96 Hours of Hindlimb Suspension-Reference-Cited by-同舟云学术

The Oxidative Phosphorylation and Cytoskeleton Proteins of Mouse Ovaries after 96 Hours of Hindlimb Suspension

Published:2023-12-12 Issue:12 Volume:13 Page:2332
ISSN:2075-1729
Container-title:Life
language:en
Short-container-title:Life

Author:

Gorbacheva Elena Yu.¹²,Sventitskaya Maria A.¹³^ORCID,Biryukov Nikolay S.¹³,Ogneva Irina V.¹³^ORCID

Affiliation:

1. Cell Biophysics Laboratory, State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe shosse, Moscow 123007, Russia

2. Gynecology Department, FGBU KB1 (Volynskaya) UDP RF, 10, Starovolynskaya Str., Moscow 121352, Russia

3. Medical and Biological Physics Department, I. M. Sechenov First Moscow State Medical University, 8-2 Trubetskaya St., Moscow 119991, Russia

Abstract

The purpose of this study was to assess oxidative phosphorylation (OXPHOS) in mouse ovaries, determine the relative content of proteins that form the respiratory chain complexes and the main structures of the cytoskeleton, and determine the mRNA of the corresponding genes after hindlimb suspension for 96 h. After hindlimb suspension, the maximum rate of oxygen uptake increased by 133% (p < 0.05) compared to the control due to the complex I of the respiratory chain. The content of mRNA of genes encoding the main components of the respiratory chain increased (cyt c by 78%, cox IV by 56%, ATPase by 69%, p < 0.05 compared with the control). The relative content of cytoskeletal proteins that can participate in the processes of transport and localization of mitochondria does not change, with the exception of an increase in the content of alpha-tubulin by 25% (p < 0.05) and its acetylated isoform (by 36%, p < 0.05); however, the mRNA content of these cytoskeletal genes did not differ from the control. The content of GDF9 mRNA does not change after hindlimb suspension. The data obtained show that short-term exposure to simulated weightlessness leads to intensification of metabolism in the ovaries.

Funder

State Scientific Center of the Russian Federation—Institute of Biomedical Problems of the Russian Academy of Sciences

Publisher

MDPI AG

Subject

Paleontology,Space and Planetary Science,General Biochemistry, Genetics and Molecular Biology,Ecology, Evolution, Behavior and Systematics

Link

https://www.mdpi.com/2075-1729/13/12/2332/pdf

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