Cell-Free DNA Hydroxymethylation in Cancer: Current and Emerging Detection Methods and Clinical Applications-Reference-Cited by-同舟云学术

Cell-Free DNA Hydroxymethylation in Cancer: Current and Emerging Detection Methods and Clinical Applications

Published:2024-09-03 Issue:9 Volume:15 Page:1160
ISSN:2073-4425
Container-title:Genes
language:en
Short-container-title:Genes

Author:

Li Janice J. N.¹^ORCID,Liu Geoffrey¹²³,Lok Benjamin H.¹²⁴^ORCID

Affiliation:

1. Department of Medical Biophysics, Temerty Faculty of Medicine, University of Toronto, Princess Margaret Cancer Research Tower, 101 College Street, Room 9-309, Toronto, ON M5G 1L7, Canada

2. Institute of Medical Science, Temerty Faculty of Medicine, University of Toronto, 1 King’s College Circle, Medical Sciences Building, Room 2374, Toronto, ON M5S 1A8, Canada

3. Department of Medical Oncology and Hematology, Princess Margaret Cancer Centre, 610 University Ave, Toronto, ON M5G 2C4, Canada

4. Radiation Medicine Program, Princess Margaret Cancer Centre, 610 University Ave, Toronto, ON M5G 2C4, Canada

Abstract

In the era of precision oncology, identifying abnormal genetic and epigenetic alterations has transformed the way cancer is diagnosed, managed, and treated. 5-hydroxymethylcytosine (5hmC) is an emerging epigenetic modification formed through the oxidation of 5-methylcytosine (5mC) by ten-eleven translocase (TET) enzymes. DNA hydroxymethylation exhibits tissue- and cancer-specific patterns and is essential in DNA demethylation and gene regulation. Recent advancements in 5hmC detection methods and the discovery of 5hmC in cell-free DNA (cfDNA) have highlighted the potential for cell-free 5hmC as a cancer biomarker. This review explores the current and emerging techniques and applications of DNA hydroxymethylation in cancer, particularly in the context of cfDNA.

Funder

Terry Fox Research Institute

Canada Foundation for Innovation

Cancer Research Society

Canadian Institutes of Health Research

National Institute of Health/National Cancer Institute

Clinical and Translational Science Center at Weill Cornell Medical Center

MSKCC

M. Qasim Choksi Chair in Lung Cancer Translational Research

Lusi Wong Family Fund

Posluns family fund

Canadian Institutes of Health Research (CIHR) Canada Graduate Scholarship—Master’s