Identification and Expression Analysis of the WOX Transcription Factor Family in Foxtail Millet (Setaria italica L.)
Author:
Nan Lizhang1, Li Yajun1, Ma Cui1, Meng Xiaowei1, Han Yuanhuai1, Li Hongying1, Huang Mingjing1, Qin Yingying2, Ren Xuemei2ORCID
Affiliation:
1. College of Agriculture, Shanxi Agricultural University, Taigu, Jinzhong 030800, China 2. College of Life Sciences, Shanxi Agricultural University, Taigu, Jinzhong 030800, China
Abstract
WUSCHEL-related homeobox (WOX) transcription factors are unique to plants and play pivotal roles in plant development and stress responses. In this investigation, we acquired protein sequences of foxtail millet WOX gene family members through homologous sequence alignment and a hidden Markov model (HMM) search. Utilizing conserved domain prediction, we identified 13 foxtail millet WOX genes, which were classified into ancient, intermediate, and modern clades. Multiple sequence alignment results revealed that all WOX proteins possess a homeodomain (HD). The SiWOX genes, clustered together in the phylogenetic tree, exhibited analogous protein spatial structures, gene structures, and conserved motifs. The foxtail millet WOX genes are distributed across 7 chromosomes, featuring 3 pairs of tandem repeats: SiWOX1 and SiWOX13, SiWOX4 and SiWOX5, and SiWOX11 and SiWOX12. Collinearity analysis demonstrated that WOX genes in foxtail millet exhibit the highest collinearity with green foxtail, followed by maize. The SiWOX genes primarily harbor two categories of cis-acting regulatory elements: Stress response and plant hormone response. Notably, prominent hormones triggering responses include methyl jasmonate, abscisic acid, gibberellin, auxin, and salicylic acid. Analysis of SiWOX expression patterns and hormone responses unveiled potential functional diversity among different SiWOX genes in foxtail millet. These findings lay a solid foundation for further elucidating the functions and evolution of SiWOX genes.
Funder
National Natural Science Foundation of China Basic Research Program of Shanxi Province Scientific and Technological Innovation Programs of Higher Education Innovation Project of Shanxi Foxtail Millet Industry Science and Technology Innovation Fund Project of Shanxi agricultural university
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