Identification of Sex-Associated Genetic Markers in Pistacia lentiscus var. chia for Early Male Detection

Author:

Stavridou Evangelia12ORCID,Karamichali Ioanna2ORCID,Siskas Evangelos1,Bosmali Irini2,Osanthanunkul Maslin34,Madesis Panagiotis25ORCID

Affiliation:

1. Department of Botany, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece

2. Laboratory of Agrobiotechnology and Molecular Plant Breeding, Institute of Applied Biosciences (INAB), Center for Research and Technology (CERTH), 57001 Thessaloniki, Greece

3. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand

4. Research Centre in Bioresources for Agriculture, Industry and Medicine, Chiang Mai University, Chiang Mai 50200, Thailand

5. Laboratory of Molecular Biology of Plants, Department of Agriculture Crop Production and Rural Environment, School of Agricultural Sciences, University of Thessaly, 38446 Volos, Greece

Abstract

Pistacia lentiscus var. chia is a valuable crop for its high-added-value mastic, a resin with proven pharmaceutical and cosmeceutical properties harvested from the male tree trunk. To achieve the maximum economic benefits from the cultivation of male mastic trees, it is important to develop early sex diagnosis molecular tools for distinguishing the sex type. Thus far, the work on sex identification has focused on Pistacia vera with promising results; however, the low transferability rates of these markers in P. lentiscus necessitates the development of species-specific sex-linked markers for P. lentiscus var. chia. To our knowledge, this is the first report regarding: (i) the development of species-specific novel transcriptome-based markers for P. lentiscus var. chia and their assessment on male, female and monoecious individuals using PCR-HRM analysis, thus, introducing a cost-effective method for sex identification with high accuracy that can be applied with minimum infrastructure, (ii) the effective sex identification in mastic tree using a combination of different sex-linked ISSR and SCAR markers with 100% accuracy, and (iii) the impact evaluation of sex type on the genetic diversity of different P. lentiscus var. chia cultivars. The results of this study are expected to provide species-specific markers for accurate sex identification that could contribute to the selection process of male mastic trees at an early stage for mass propagation systems and to facilitate future breeding efforts related to sex-linked productivity and quality of mastic resin.

Publisher

MDPI AG

Reference55 articles.

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2. (2023, March 25). European Commission. Available online: https://ec.europa.eu/agriculture/eambrosia/geographical-indications-register/details/EUGI00000013337.

3. (2023, March 25). Intangible Cultural Heritage of Humanity (UNESCO) 2014. Available online: https://ich.unesco.org/en/RL/know-how-of-cultivating-mastic-on-the-island-of-chios-00993.

4. Pistachio (Pistacia Spp.) Breeding;Jain;Advances in Plant Breeding Strategies: Nut and Beverage Crops,2019

5. Systematics and Evolution Pistacia lentiscus Cv. Chia (Anacardiaceae) on Chios Island;Browicz;Plant Syst. Evol.,1987

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