Analysis of Transcriptomic Differences in the Ovaries of High- and Low-Laying Ducks
Author:
Chang Yuguang1, Guo Rongbing2, Zeng Tao1, Sun Hanxue1, Tian Yong1, Han Xue3, Cao Yongqing1, Xu Ligen1, Duan Mingcai1, Lu Lizhi1, Chen Li1
Affiliation:
1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China 2. College of Animal Sciences and Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China 3. Institute of Animal Husbandry and Veterinary Science, Guizhou Academy of Agricultural Sciences, Guiyang 550000, China
Abstract
The egg-laying performance of Shan Ma ducks (Anas Platyrhynchos) is a crucial economic trait. Nevertheless, limited research has been conducted on the egg-laying performance of this species. We examined routine blood indicators and observed higher levels of metabolic and immune-related factors in the high-egg-production group compared with the low-egg-production group. Furthermore, we explored the ovarian transcriptome of both high- and low-egg-production groups of Shan Ma ducks using Illumina NovaSeq 6000 sequencing. A total of 1357 differentially expressed genes (DEGs) were identified, with 686 down-regulated and 671 up-regulated in the high-egg-production (HEP) ducks and low-egg-production (LEP) ducks. Several genes involved in the regulation of ovarian development, including neuropeptide Y (NPY), cell cycle protein-dependent kinase 1 (CDK1), and transcription factor 1 (E2F1), exhibited significant differential expressions at varying stages of egg production. Pathway functional analysis revealed that the DEGs were primarily associated with the steroid biosynthesis pathway, and the neuroactive ligand–receptor interaction pathway exhibited higher activity in the HEP group compared to the LEP group. This study offers valuable information about and novel insights into high egg production.
Funder
National Key Research and Development Program of China China Agriculture Research System of MOF and MARA Zhejiang Province Agricultural New Breed Breeding Major Science and Technology Special Project Qiankehe Achievement
Subject
Genetics (clinical),Genetics
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