Identification of Barley yellow mosaic virus Isolates Breaking rym3 Resistance in Japan

Author:

Zhu Hongjing12ORCID,Okiyama Takeshi3,Mishina Kohei1,Kikuchi Shinji2,Sassa Hidenori2,Komatsuda Takao4,Kato Tsuneo3,Oono Youko12

Affiliation:

1. Institute of Crop Science, National Agriculture and Food Research Organization (NARO), 2-1-2 Kannondai, Tsukuba 305-8602, Japan

2. Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo 271-8510, Japan

3. Tochigi Prefectural Agricultural Experiment Station, Kawaraya-cho 1080, Utsunomiya 320-0002, Japan

4. Shandong Academy of Agricultural Sciences (SAAS), Crop Research Institute, 202 Gongyebei Road, Licheng District, Jinan 250100, China

Abstract

In early spring 2018, significant mosaic disease symptoms were observed for the first time on barley leaves (Hordeum vulgare L., cv. New Sachiho Golden) in Takanezawa, Tochigi Prefecture, Japan. This cultivar carries the resistance gene rym3 (rym; resistance to yellow mosaic). Through RNA-seq analysis, Barley yellow mosaic virus (BaYMV-Takanezawa) was identified in the roots of all five plants (T01–T05) in the field. Phylogenetic analysis of RNA1, encompassing known BaYMV pathotypes I through V, revealed that it shares the same origin as isolate pathotype IV (BaYMV-Ohtawara pathotype). However, RNA2 analysis of isolates revealed the simultaneous presence of two distinct BaYMV isolates, BaYMV-Takanezawa-T01 (DRR552862, closely related to pathotype IV) and BaYMV-Takanezawa-T02 (DRR552863, closely related to pathotype III). The amino acid sequences of the BaYMV-Takanezawa isolates displayed variations, particularly in the VPg and N-terminal region of CP, containing mutations not found in other domains of the virus genome. Changes in the CI (RNA1 amino acid residue 459) and CP (RNA1 amino acid residue 2138) proteins correlated with pathogenicity. These findings underscore the importance of monitoring and understanding the genetic diversity of BaYMV for effective disease management strategies in crop breeding.

Funder

Ministry of Agriculture, Forestry and Fisheries (MAFF) of Japan

JSPS KAKENHI

Government of Japan

Publisher

MDPI AG

Reference59 articles.

1. Polymyxa graminis and the cereal viruses it transmits: A research challenge;Kanyuka;Mol. Plant Pathol.,2003

2. Friedt, W., Kaiser, R., Götz, R., and Umbach, H. (1987). Proceedings of a Conference at the University of St Andrews, Association of Applied Biologists, AAB Office, c/o Institute of Horticultural Research, Wellesbourne.

3. Breeding for resistance to yellow mosaic disease in malting barley;Kobayashi;Barley Genet. Newsl.,1987

4. Development of a two-rowed malting barley cultivar resistant to barley yellow mosaic;Seko;Jpn. Agric. Res. Q.,1987

5. Effects of barley yellow mosaic virus infection on yield and malting quality of barley;Yamaguchi;Bull. Tochigi Prefect. Agric. Exp. Stn.,2002

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3