Identification and Potential Roles of Human MicroRNAs in Ebola Virus Infection and Disease Pathogenesis

Author:

Mensah-Bonsu Melvin1ORCID,Doss Christopher2,Gloster Clay3,Muganda Perpetua4ORCID

Affiliation:

1. Applied Science and Technology Ph.D. Program, North Carolina A&T State University, Greensboro, NC 27411, USA

2. Department of Electrical and Computer Engineering, North Carolina A&T State University, Greensboro, NC 27411, USA

3. Department of Computer Systems Technology, North Carolina A&T State University, Greensboro, NC 27411, USA

4. Department of Biology, North Carolina A&T State University, Greensboro, NC 27411, USA

Abstract

Ebola virus (EBOV) is a highly pathogenic virus that causes a severe illness called Ebola virus disease (EVD). EVD has a high mortality rate and remains a significant threat to public health. Research on EVD pathogenesis has traditionally focused on host transcriptional responses. Limited recent studies, however, have revealed some information on the significance of cellular microRNAs (miRNAs) in EBOV infection and pathogenic mechanisms, but further studies are needed. Thus, this study aimed to identify and validate additional known and novel human miRNAs in EBOV-infected adult retinal pigment epithelial (ARPE) cells and predict their potential roles in EBOV infection and pathogenic mechanisms. We analyzed previously available small RNA-Seq data obtained from ARPE cells and identified 23 upregulated and seven downregulated miRNAs in the EBOV-infected cells; these included two novel miRNAs and 17 additional known miRNAs not previously identified in ARPE cells. In addition to pathways previously identified by others, these miRNAs are associated with pathways and biological processes that include WNT, FoxO, and phosphatidylinositol signaling; these pathways were not identified in the original study. This study thus confirms and expands on the previous study using the same datasets and demonstrates further the importance of human miRNAs in the host response and EVD pathogenesis during infection.

Funder

Department of Defense

Publisher

MDPI AG

Reference72 articles.

1. Ebola virus disease: A narrative review;Letafati;Microb. Pathog.,2023

2. Ebola haemorrhagic fever;Feldmann;Lancet,2011

3. (2023, December 28). Center for Disease Control and Prevention Ebola Disease, Available online: https://www.cdc.gov/vhf/ebola/index.html.

4. Ebola virus disease;Jacob;Nat. Rev. Dis. Primers,2020

5. Diagnostics of Ebola virus;Bettini;Front. Public Health,2023

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3