Determination of Fumonisins in Grains and Poultry Feedstuffs in Croatia: A 16-Year Study

Abstract

Fumonisins are a group of closely related mycotoxins produced by Fusarium, Alternaria alternata and Aspergillus species. Their occurrence is correlated with various factors during growth, processing and storage. Fumonisins occurrence data in the literature mainly include the B group of fumonisins (FB1 & FB2) in raw materials, showing high frequency of positive samples in a wide range of concentrations. In this study, a total of 933 grains (63.7%) and poultry feed (36.3%) samples, collected in the 16-year period (2006–2021), were analysed with commercial enzyme-linked-immunosorbent assay for detection of three fumonisins (FB1, FB2 & FB3). All positive and suspect samples were confirmed with high-performance-liquid-chromatography method with fluorescence detection. Overall, we have determined high occurrence of FBs in grains and poultry feed in all tested years, while the lowest occurrence was determined in 2019, followed by 2009 and 2008. Although, contamination levels varied from year-to-year, majority of analyzed samples in all tested years were around 1 mg/kg, while the maximum values varied from 3 mg/kg to 22.23 mg/kg. This study highlights the importance of regular monitoring of raw materials and understanding of the fate of FBs in the food chain in order to avoid undesirable health effects in animals and accompanied economic losses.