Selenium-Fortified Kombucha–Pollen Beverage by In Situ Biosynthesized Selenium Nanoparticles with High Biocompatibility and Antioxidant Activity
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Published:2023-09-02
Issue:9
Volume:12
Page:1711
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ISSN:2076-3921
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Container-title:Antioxidants
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language:en
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Short-container-title:Antioxidants
Author:
Tritean Naomi12ORCID, Dima Ștefan-Ovidiu1ORCID, Trică Bogdan13ORCID, Stoica Rusăndica1, Ghiurea Marius1ORCID, Moraru Ionuț4, Cimpean Anisoara2ORCID, Oancea Florin15ORCID, Constantinescu-Aruxandei Diana1ORCID
Affiliation:
1. Bioresources, Polymers and Analysis Departments, National Institute for Research & Development in Chemistry and Petrochemistry—ICECHIM, Splaiul Independenței No. 202, Sector 6, 060021 Bucharest, Romania 2. Faculty of Biology, University of Bucharest, Splaiul Independentei No. 91-95, 050095 Bucharest, Romania 3. Postdoctoral School, National University of Science and Technology Politehnica of Bucharest, Splaiul Independenței No. 313, 060042 Bucharest, Romania 4. Medica Laboratories, Str. Frasinului nr. 11, 075100 Otopeni, Romania 5. Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Mărăști Blv. No. 59, Sector 1, 011464 Bucharest, Romania
Abstract
Biogenic selenium nanoparticles (SeNPs) have been shown to exhibit increased bioavailability. Fermentation of pollen by a symbiotic culture of bacteria and yeasts (SCOBY/Kombucha) leads to the release of pollen content and enhances the prebiotic and probiotic effects of Kombucha. The aim of this study was to fortify Kombucha beverage with SeNPs formed in situ by Kombucha fermentation with pollen. Response Surface Methodology (RSM) was used to optimize the biosynthesis of SeNPs and the pollen-fermented Kombucha beverage. SeNPs were characterized by Transmission electron microscopy energy-dispersive X-ray spectroscopy (TEM-EDX), Fourier-transform infrared spectroscopy (FTIR), Dynamic light scattering (DLS), and Zeta potential. The pollen-fermented Kombucha beverage enriched with SeNPs was characterized by measuring the total phenolic content, antioxidant activity, soluble silicon, saccharides, lactic acid, and the total content of Se0. The polyphenols were identified by liquid chromatography–mass spectrometry (LC-MS). The pollen and the bacterial (nano)cellulose were characterized by scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX), FTIR, and X-Ray diffraction (XRD). We also assessed the in vitro biocompatibility in terms of gingival fibroblast viability and proliferation, as well as the antioxidant activity of SeNPs and the pollen-fermented Kombucha beverage enriched with SeNPs. The results highlight their increased biological performance in this regard.
Subject
Cell Biology,Clinical Biochemistry,Molecular Biology,Biochemistry,Physiology
Reference104 articles.
1. Dietary supplements and nutraceuticals market growth during the coronavirus pandemic—Implications for consumers and regulatory oversight;Lordan;PharmaNutrition,2021 2. Aylanc, V., Larbi, S., Calhelha, R., Barros, L., Rezouga, F., Rodríguez-Flores, M.S., Seijo, M.C., El Ghouizi, A., Lyoussi, B., and Falcão, S.I. (2023). Evaluation of Antioxidant and Anticancer Activity of Mono- and Polyfloral Moroccan Bee Pollen by Characterizing Phenolic and Volatile Compounds. Molecules, 28. 3. Gercek, Y.C., Celik, S., and Bayram, S. (2022). Screening of Plant Pollen Sources, Polyphenolic Compounds, Fatty Acids and Antioxidant/Antimicrobial Activity from Bee Pollen. Molecules, 27. 4. Oroian, M., Dranca, F., and Ursachi, F. (2022). Characterization of Romanian Bee Pollen—An Important Nutritional Source. Foods, 11. 5. Uțoiu, E., Matei, F., Toma, A., Diguță, C.F., Ștefan, L.M., Mănoiu, S., Vrăjmașu, V.V., Moraru, I., Oancea, A., and Israel-Roming, F. (2018). Bee Collected Pollen with Enhanced Health Benefits, Produced by Fermentation with a Kombucha Consortium. Nutrients, 10.
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