Optimizing Anesthetic Practices for Mud Crab: A Comparative Study of Clove Oil, MS-222, Ethanol, and Magnesium Chloride
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Published:2023-12-16
Issue:12
Volume:12
Page:2124
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ISSN:2076-3921
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Container-title:Antioxidants
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language:en
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Short-container-title:Antioxidants
Author:
Zhu Lulu1234, Qi Shanshan1234, Shi Ce1234ORCID, Chen Shujian1234, Ye Yangfang234, Wang Chunlin234, Mu Changkao234, Li Ronghua234ORCID, Wu Qingyang234, Wang Xiaopeng234ORCID, Zhou Yueyue234
Affiliation:
1. Marine Economic Research Center, Donghai Academy, Ningbo University, Ningbo 315000, China 2. Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo 315000, China 3. Key Laboratory of Green Mariculture (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo 315000, China 4. Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo 315000, China
Abstract
Anesthesia serves as an effective method to mitigate the stress response in aquatic animals during aquaculture and product transportation. In this study, we assessed the anesthetic efficacy of clove oil, tricaine methane-sulfonate (MS-222), ethanol, and magnesium chloride by anesthesia duration, recovery time, 24-hour survival rate, and the behavior of mud crabs (Scylla paramamosain). Additionally, the optimal anesthetic concentration for varying body weights of mud crabs was also investigated. The results revealed that clove oil emerged as the optimal anesthetic for mud crabs, with a 24-hour survival rate surpassing those observed in MS-222 and magnesium chloride treatments. Ethanol caused amputation and hyperactivity in mud crabs. Regression analyses between the optimal anesthetic concentration of clove oil and the weight categories of 0.03–27.50 g and 27.50–399.73 g for mud crabs yielded the following equations: y = 0.0036 x3 − 0.1629 x2 + 1.7314 x + 4.085 (R2 = 0.7115) and y = 0.0437 x + 2.9461 (R2 = 0.9549). Clove oil exhibited no significant impact on serum cortisol, glucose, lactate content, aspartate aminotransferase (AST), alanine aminotransferase (ALT) activities, or superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) levels in mud crabs across different treatment groups. Anesthesia induced by clove oil in mud crabs resulted in an increase in inhibitory neurotransmitters such as glycine. However, the recovery from anesthesia was associated with elevated levels of the excitatory neurotransmitters L-aspartic acid and glutamate. In conclusion, clove oil proves to be a safe and optimal anesthetic agent for mud crabs, exerting no physiological stress on the species.
Funder
Province Key Research and Development Program of Zhejiang Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties National Natural Science Foundation of China 2025 Technological Innovation for Ningbo Agriculture Research System of China of MOF and MARA K.C. Wong. Magna Fund of Ningbo University Scientific Research Foundation of the Graduate School of Ningbo University
Subject
Cell Biology,Clinical Biochemistry,Molecular Biology,Biochemistry,Physiology
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