Development and Recovery of Liver Injury in Piglets by Incremental Injection of LPS
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Published:2023-05-24
Issue:6
Volume:12
Page:1143
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ISSN:2076-3921
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Container-title:Antioxidants
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language:en
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Short-container-title:Antioxidants
Author:
Duan Geyan12, Huang Pan1, Zheng Changbing3, Zheng Jie12, Yu Jiayi12, Zhang Peiwen3, Wan Mengliao3, Li Fengna12ORCID, Guo Qiuping12, Yin Yulong123, Duan Yehui12ORCID
Affiliation:
1. CAS Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, China 2. University of Chinese Academy of Sciences, Beijing 100049, China 3. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
Abstract
This study aimed to explore the effects of the incremental injection of lipopolysaccharide (LPS) on liver histopathology, inflammation, oxidative status, and mitochondrial function in piglets. Forty healthy Duroc × Landrace × Yorkshire castrated boars (21 ± 2 days old, weight 6.84 ± 0.11 kg) were randomly assigned to five groups (n = 8) and then slaughtered on days 0 (group 0, without LPS injection), 1 (group 1), 5 (group 5), 9 (group 9), and 15 (group 15) of LPS injection, respectively. The results showed that, compared to the piglets without LPS injection, LPS injection caused liver injury in the early phase, as manifested by the increased activities of serum liver injury-related parameters (aspartate amino transferase, alanine aminotransferase, alkaline phosphatase, cholinesterase, and total bile acid) on day 1, and impaired liver morphology (disordered hepatic cell cord arrangement, dissolved and vacuolized hepatocytes, karyopycnosis, and inflammatory cell infiltration and congestion) on days 1 and 5. Meanwhile, LPS injection caused liver inflammation, oxidative stress, and mitochondrial dysfunction on days 1 and 5, as reflected by the upregulated mRNA expression of TNF-α, IL-6, IL-1β, TLR4, MyD88, and NF-κB; increased MPO and MDA content; and impaired mitochondrial morphology. However, these parameters were ameliorated in the later phase (days 9~15). Taken together, our data indicate that the incremental injection of the LPS-induced liver injury of piglets could be self-repaired.
Funder
National Natural Science Foundation of China Changsha Natural Science Funds for Distinguished Young Scholar science and technology innovation Program of Hunan Province Natural Science Foundation of Hunan Province Natural Science Foundation of Guangxi Province National Key Research and Development Programs of China China Agriculture Research System of MOF and MARA
Subject
Cell Biology,Clinical Biochemistry,Molecular Biology,Biochemistry,Physiology
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