Antibacterial Activity of ZnSe, ZnSe-TiO2 and TiO2 Particles Tailored by Lysozyme Loading and Visible Light Irradiation
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Published:2023-03-10
Issue:3
Volume:12
Page:691
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ISSN:2076-3921
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Container-title:Antioxidants
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language:en
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Short-container-title:Antioxidants
Author:
Anastasescu Crina1, Neagu Simona2ORCID, Preda Silviu1ORCID, Culita Daniela1ORCID, Stancu Mihaela2, Banciu Cristian2, Munteanu Cornel1, Bratan Veronica1, Calderon-Moreno Jose Maria1, State Razvan1, Anastasescu Mihai1ORCID, Enache Madalin2ORCID, Balint Ioan1, Zaharescu Maria1ORCID
Affiliation:
1. “Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania 2. Institute of Biology of Romanian Academy, 296 Splaiul Independenţei, 060031 Bucharest, Romania
Abstract
ZnSe, ZnSe-TiO2 microspheres and nanostructured TiO2 obtained by hydrothermal and sol–gel methods were tested against Staphylococcus aureus ATCC 25923 and Micrococcus lysodeikticus ATCC 4698 before and after lysozyme (Lys) loading. Morphological characterization of inorganic matrices and hybrid organic–inorganic complexes were performed by microscopy techniques (SEM, AFM and Dark Field Hyperspectral Microscopy). Light absorption properties of ZnSe, ZnSe-TiO2 and TiO2 powders were assessed by UV–visible spectroscopy and their ability to generate reactive oxygen species (•OH and O2•−) under visible light irradiation was investigated. Antibacterial activity of ZnSe, ZnSe-TiO2, TiO2, Lys/ZnSe, Lys/ZnSe-TiO2 and Lys/TiO2 samples under exposure to visible light irradiation (λ > 420 nm) was tested against Staphylococcus aureus and Micrococcus lysodeikticus and correlated with ROS photogeneration.
Subject
Cell Biology,Clinical Biochemistry,Molecular Biology,Biochemistry,Physiology
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