Green Synthesis of Gold Nanoparticles with Curcumin or Açai in the Tissue Repair of Palatal Wounds
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Published:2023-08-07
Issue:8
Volume:12
Page:1574
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ISSN:2076-3921
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Container-title:Antioxidants
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language:en
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Short-container-title:Antioxidants
Author:
Thirupathi Anand1ORCID, Guzzatti Morgana Francisco Machado2, Corrêa Maria Eduarda Anastácio Borges2, Venturini Ligia Milanez2, Casagrande Laura de Roch2, Lima Igor Ramos2, Da Costa Camila2, De Pieri Ellen2, Tietbohl Lariani Tamires Witt2, Feuser Paulo Emilio2, Machado-de-Ávila Ricardo Andrez2, Gu Yaodong1ORCID, Silveira Paulo Cesar Lock2
Affiliation:
1. Research Academy of Medicine Combining Sports, Ningbo No. 2 Hospital, Ningbo 315099, China 2. Laboratory of Experimental Physiopathology, Program of Postgraduate in Science of Health, Universidade do Extremo-Sul Catarinense, Criciúma 88806-000, SC, Brazil
Abstract
This study aimed to evaluate and compare the effects of treatment with gold nanoparticles (GNPs) reduced with Curcumin (Curcuma longa L.) or Açai (Euterpe oleracea) to a standard commercial treatment of the pharmacological type (Omcilon®) and an electrophysical agent (photobiomodulation) in the palatal wounds of rats. As for the in vitro assay, a cell viability test was performed to assess the toxicity of the synthesized nanoparticles. In vivo assay: 60 Wistar rats were divided into five groups (n = 12): I. Palatal Wound (PW); II. PW + Photobiomodulation (PBM); III. PW + Omcilon®; IV. PW + GNPs-Cur (0.025 mg/mL); V. PW + GNPs-Açai (0.025 mg/mL). Animals were first anesthetized, and circular lesions in the palatine mucosa were induced using a 4 mm-diameter punch. The first treatment session started 24 h after the injury and occurred daily for 5 days. The animals were euthanized, and the palatal mucosa tissue was removed for histological, biochemical, and molecular analysis. GNPs-Açai were able to significantly reduce pro-inflammatory cytokines and increase anti-inflammatory ones, reduce oxidant markers, and reduce inflammatory infiltrate while increasing the collagen area and contraction rate of the wound, along with an improved visual qualification. The present study demonstrated that the proposed therapies of GNPs synthesized greenly, thus associating their effects with those of plants, favor the tissue repair process in palatal wounds.
Funder
Conselho Nacional de Desenvolvimento Científico e Tecnológico Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina Universidade do Extremo Sul Catarinense Research Academy of Medicine Combining Sports, Ningbo NINGBO Leading Medical & Health Discipline
Subject
Cell Biology,Clinical Biochemistry,Molecular Biology,Biochemistry,Physiology
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