Effect of Waterlogging Stress on Leaf Anatomical Structure and Ultrastructure of Phoebe sheareri Seedlings

Abstract

Phoebe sheareri is an excellent roadside tree with a wide distribution range and high ornamental value. Excessive moisture can affect the external morphology, the microstructure, and the ultrastructure of the leaf. Little is known at present regarding the leaf structure of P. sheareri under waterlogging stress. In this paper, the external morphology of leaves, the microstructure of leaf epidermis, and the ultrastructure of mesophyll cells of P. sheareri seedlings under waterlogging stress and drainage were dynamically observed. Waterlogging stress contributed to the yellowing and wilting of P. sheareri seedling leaves, the gradual closing of leaf epidermal stomata, increasing density of leaf stomata, gradual loosening of the arrangement of leaf cell structure, and merging of leaf palisade tissue cells. Waterlogging stress forced the structure of the chloroplast membranes to blur, gradually causing swelling, and deformation, with plasmolysis occurring in severe cases. During waterlogging, the basal lamellae were disorganized, and the mitochondrial membrane structure was damaged. The damaged state of the leaves was not relieved after drainage. Waterlogging stress not only inhibited the growth of leaves, but also accelerated the closure of stomata, disordered the arrangement of palisade tissue and spongy tissue gradually, and damaged the internal organelles of mesophyll cells.