Molecular Evidence for the Presence of Wisteria Vein Mosaic Virus in Italy: Shedding Light on Genetic Diversity and Evolutionary Dynamics of Virus Geographic Populations

Abstract

Wisteria vein mosaic virus (WVMV) is a member of the genus Potyvirus, found to be associated with the Wisteria mosaic disease (WMD). In 2022, severe symptoms resembling WMD were observed on the foliage of a Chinese wisteria (Wisteria sinensis) tree growing in Apulia (Italy). The presence of WVMV was ascertained by RT-PCR using the universal degenerate primers NIb2F/NIb3R in the NIb gene. Next, we derived the complete sequence of the CP gene. MAFFT pairwise alignment of the two target regions showed a high amino acid sequence identity of isolate Bari with other WVMV isolates, confirming they belonged to the same species. NIb phylogenetic analysis placed WVMV Bari within the lineage identified in the genus Potyvirus as the bean common mosaic virus supergroup. Closer analysis based on the CP gene showed that WVMV Bari was part of a sub-clade gathering all WVMV isolates within a larger clade clustering closely related species. An exploratory analysis of the genetic diversity and possible mechanisms underlying the evolution of geographic populations of WVMV was attempted. The analysis was based on the N-terminal coding region of CP, available for 20 isolates from Europe, Asia, and Oceania. A high diversity, mainly found in the European population, led to the identification of 16 different haplotypes. Based on the dN/dS ratio, the target region appears to be under negative selective pressure. Neutrality tests indicated a potential population expansion in Europe and a recent decrease in Asia. The two populations exhibited a high level of gene flow. We found that WVMV Bari may have originated from a recombination event in the NIb gene. To date, the presence of WVMV had never been reported on W. sinensis in Italy, and no molecular information was available on any Italian isolate. Our report draws attention to the further spread of WVMV in the European territory and its rising threat to the ornamental nursery sector.