Insights into the Belowground Biodiversity and Soil Nutrient Status of an Organic Apple Orchard as Affected by Living Mulches

Abstract

The impact of living mulches established with three officinal plants (Alchemilla vulgaris, Fragaria vesca and Mentha x piperita) on the soil bacterial microbiome and activity, the nematodes population, and the nutrient status of an organic apple orchard was assessed. The composition and diversity of the bacterial communities were differentially modified by living mulches. The activity of the bacterial microbiome associated with F. vesca was higher and utilized more C sources in comparison to other treatments. The combined analysis of the core bacterial microbiome and metabolic activity pointed to a potential effect of F. vesca on different levels of the soil’s trophic network. The living mulches did not affect the overall number of nematodes, but in some cases, they modified the structure of the population: F. vesca induced the highest share of bacteria feeders and the lowest number of herbivores and fungal feeders. The living mulches modified the availability of some nutrients and the pH. Multivariate analysis of the whole dataset showed several potential inter-dependencies between the assessed parameters that are worthy of further study. In conclusion, the introduction of multifunctional living mulches based on officinal plants induced changes to the soil’s genetic and functional biodiversity and chemical properties. These modifications could deliver ecosystem services particularly relevant to organic apple orchards.