Assessment of the Physiological Values and the Reference Histological Profile Related to Sex Steroids in Veal Calves

Author:

Pezzolato Marzia1ORCID,Biasibetti Elena1,Gili Marilena1,Maurella Cristiana1ORCID,Benedetto Alessandro1ORCID,Marturella Marianna1,Ostorero Federica1,Bozzo Giancarlo2ORCID,Bellino Claudio3ORCID,D’angelo Antonio3ORCID,Valentini Flaminia3,Roberti Francesca4,Fiori Maurizio5,Stacchini Paolo5,Bozzetta Elena1

Affiliation:

1. Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Via Bologna 148, 10154 Torino, Italy

2. Department of Veterinary Medicine, University of Bari ‘Aldo Moro’, 70010 Bari, Italy

3. Dipartimento di Scienze Veterinarie, Università degli Studi di Torino, Largo Paolo Braccini 2, 10095 Torino, Italy

4. Direzione Generale per L’igiene e la Sicurezza Degli Alimenti e la Nutrizione, Ufficio 8 Ministero della Salute, Via G. Ribotta, 5, 00144 Roma, Italy

5. Dipartimento Sicurezza Alimentare, Nutrizione e Sanità Pubblica Veterinaria, Istituto Superiore di Sanit Viale Regina Elena, 299, 00161 Rome, Italy

Abstract

Among forbidden substances included in the European Union legislation, endogenous steroids constitute a challenge in the framework of veterinary Official Monitoring Plans. They can be naturally present in body fluids at variable levels depending on the species, sex and age of the animals. Considering the significant advances achieved in breeding conditions and in the selection of producing traits in meat cattle, the aim of this study was to verify by analytical method, in veal calves housed under controlled conditions, if the level of natural steroids hormones assumed as physiological are still actual. The second aim of the study was to verify if the normal histological pattern of growth promoters in target organs is influenced accordingly. Bovine male sex organs are currently analysed in the frame of the Italian histological plan to monitor illicit treatments trend, highlighting microscopic, induced alterations. The levels of 17β-estradiol and progesterone residues resulted under the Limit of Quantitation of the approved official methods and the level of testosterone resulted below the level stated in the Italian Ministerial Decree in force. Male target organs appeared within the limits of the standard histological features.

Funder

Italian Ministry of Health

Publisher

MDPI AG

Subject

Plant Science,Agronomy and Crop Science,Food Science

Reference32 articles.

1. (1988). Council Directive 88/146/EEC of 7 March 1988 Prohibiting the Use in Livestock (Arming of Certain Substances Having a Hormonal Action (Standard No. Council Directive 88/146/EEC).

2. (1996). Council Directive 96/23/EC of 29 April 1996 on Measures to Monitor Certain Substances and Residues Thereof in Live Animals and Animal Products and Repealing Directives 85/358/EEC and 86/469/EEC and Decisions 89/187/EEC and 91/664/EEC (Standard No. Council Directive 96/23/EC of 29 April 1996).

3. EU (2017). Regulation (EU) 2017/625 of the European Parliament and of the Council of 15 March 2017 on Official Controls and Other Official Activities Performed to Ensure the Application of Food and Feed Law, Rules on Animal Health and Welfare, Plant Health and Plant, Official Journal of the European Communities.

4. Plasma biomarker profiling in the detection of growth promoter use in calves;Mooney;Biomarkers,2008

5. Effect-based proteomic detection of growth promoter abuse;McGrath;Anal. Bioanal. Chem.,2013

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