New Insights on Coding Mutations and mRNA Levels of Candidate Genes Associated with Diarrhea Susceptibility in Baladi Goat

Abstract

The purpose of this investigation was to examine mutations and mRNA levels of potential genes linked to diarrhea susceptibility in order to assess the health status of diarrheic kids of Baladi goats. One hundred female Baladi kids (35 diarrheic and 65 apparently healthy) were used. PCR-DNA sequencing was conducted for TMED1, CALR, FBXW9, HS6ST3, SMURF1, KPNA7, FBXL2, PIN1, S1PR5, ICAM1, EDN1, MAPK11, CSF1R, LRRK1, and CFH markers revealed nucleotide sequence variants in the frequency of distribution of all detected SNPs (p ˂ 0.05) between healthy and affected kids. Chi-square analysis showed a significant difference between resistant and affected animals. Gene expression profile revealed that TMED1, CALR, FBXW9, HS6ST3, SMURF1, KPNA7, FBXL2, PIN1, S1PR5, ICAM1, EDN1, MAPK11, CSF1R and LRRK1 were significantly up-regulated in diarrheic kids than resistant ones. Meanwhile, CFH gene elicited an opposite trend. On the mRNA levels of the examined indicators, there was a substantial interaction between the type of gene and diarrhea resistance/susceptibility. The findings could support the importance of nucleotide variations and the expression pattern of the examined genes as biomarkers for diarrhea resistance/susceptibility and offer a useful management strategy for Baladi goats.