Heterotrophic and Photoautotrophic Media Optimization Using Response Surface Methodology for the Novel Microalga Chlorococcum amblystomatis

Author:

Correia Nádia123ORCID,Pereira Hugo4,Schulze Peter S. C.4,Costa Monya M.4,Santo Gonçalo E.2ORCID,Guerra Inês2,Trovão Mafalda24ORCID,Barros Ana2,Cardoso Helena2,Silva Joana L.2,Gouveia Luísa34ORCID,Varela João14ORCID

Affiliation:

1. CCMAR—Centro de Ciências do Mar, Campus de Gambelas, University of Algarve, 8005-139 Faro, Portugal

2. Allmicroalgae Natural Products S.A., Research and Development Department-Rua 25 de Abril, 2445-413 Alcobaça, Portugal

3. LNEG—Laboratório Nacional de Energia e Geologia I.P., Bioenergy and Biorefineries Department, Estrada do Paço do Lumiar 22, 1649-038 Lisbon, Portugal

4. GreenCoLab-Associação Oceano Verde, Campus de Gambelas, Universidade do Algarve, 8005-139 Faro, Portugal

Abstract

The nutritional requirements of novel microalgal strains are key for their effective cultivation and metabolite content. Therefore, the optimization of heterotrophic and photoautotrophic culture media is crucial for novel Chlorococcum amblystomatis growth. Heterotrophic and photoautotrophic biomass samples were characterized to identify the differences between their heterotrophic and photoautotrophic biomass composition and their biotechnological potential. Media optimization through surface response methodology led to 44.9 and 51.2% increments in C. amblystomatis-specific growth rates under heterotrophic and photoautotrophic growth, respectively. This microalga registered high protein content (61.49–73.45% dry weight), with the highest value being observed in the optimized photoautotrophic growth medium. The lipid fraction mainly constituted polyunsaturated fatty acids, ranging from 44.47 to 51.41% for total fatty acids (TFA) in cells under heterotrophy. However, these contents became significantly higher (70.46–72.82% TFA) in cultures cultivated under photoautotrophy. An interesting carotenoids content was achieved in the cultures grown in optimized photoautotrophic medium: 5.84 mg·g−1 β-carotene, 5.27 mg·g−1 lutein, 3.66 mg·g−1 neoxanthin, and 0.75 mg·g−1 violaxanthin. Therefore, C. amblystomatis demonstrated an interesting growth performance and nutritional profile for food supplements and feed products that might contribute to meeting the world’s nutritional demand.

Funder

FCT-Foundation for Science and Technology

European Union’s Horizon 2020 research and innovation program

Portuguese national budget P2020

Publisher

MDPI AG

Subject

Fluid Flow and Transfer Processes,Computer Science Applications,Process Chemistry and Technology,General Engineering,Instrumentation,General Materials Science

Reference47 articles.

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